Mechanism of action of Radix Ranunculi Ternati in treatment of hepatocellular carcinoma:A study based on GEO database and network pharmacology
Objective:To investigate the mechanism of action of Radix Ranunculi Ternati in the treatment of hepatocellular carcinoma(HCC)based on GEO database and network pharmacology.Methods:HERB database,SwissADME,and SwissTargetPrediction were used to obtain the effective constituents and action targets of Radix Ra-nunculi Ternati.GEO database was used to obtain the differentially expressed genes between the patients with nor-mal liver tissue and the patients with HCC,and GeneCards,DisGeNET,and GEO database were used to obtain the disease targets of HCC and the intersecting genes of the disease and the components.Cytoscape 3.9.0 was used to construct a component-disease network and perform a topological analysis,and David database was used to perform gene ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)path-way enrichment analysis.Results:There were 36 active components of Radix Ranunculi Ternati,with 544 potential targets and 1 950 differentially expressed genes.The core targets included vascular endothelial growth factor A,sig-nal transducer and activator of transcription 3,caspase 3,non-receptor tyrosine kinase,hypoxia-inducible factor-1α,and epidermal growth factor receptor.GO functional enrichment analysis showed that the biological processes in-volved response to foreign body stimulation,positive regulation of mitogen-activated protein kinase cascades,and the transmembrane receptor protein tyrosine kinase signaling pathway;cellular components mainly included cytoplasm,cell nucleus,and exosome;molecular functions mainly included protein binding,enzyme activity,and deoxyribonu-cleic acid binding.The KEGG pathways mainly involved cancer,phosphatidylinositol 3-kinase/protein kinase-B,prolactin,hypoxia-inducible factor 1,and hepatitis B.Conclusion:Radix Ranunculi Ternati has the features of mul-tiple components,targets,and pathways in the treatment of HCC,which provides a theoretical basis for subsequent studies.
hepatocellular carcinomaRadix Ranunculi TernatiGEO databasenetwork pharmacologymo-lecular dockingmechanism of action
万方数据
维普
