Effect of Yimu Suogong Granule on T-helper type 1/T-helper type 2 subsets and related contraction factors in the uterus of rats with incomplete medical abortion
Objective:To investigate the effect of Yimu Suogong Granule on T-helper type 1/T-helper type 2(Th1/Th2)subsets and related contraction factors in the uterus of rats with incomplete medical abortion,as well as the mechanism of action of Yimu Suogong Granule in improving abnormal vaginal bleeding after medical abortion.Methods:A total of 9 female rats were selected as blank group,and the remaining female rats were caged with mature male rats to es-tablish a model of incomplete abortion during early pregnancy.After successful modeling,the female rats were divided into model group with 10 rats,control group with 9 rats,low-dose Yimu Suogong Granule group with 10 rats,middle-dose Yimu Suogong Granule group with 9 rats,and high-dose Yimu Suogong Granule group with 9 rats.The rats in the blank group and the model group were given animal drinking water 1 mL/100 g by gavage for 7 consecutive days,and those in the low-,middle-,and high-dose Yimu Suogong Granule groups were given Yimu Suogong Granule by gavage at a dose of 1.56 g/kg,3.13 g/kg,and 6.25 g/kg,respectively,by gavage for 7 consecutive days.The rats in the control group were given injection of oxytocin 0.9 U/kg for 3 consecutive days.The rats were observed in terms of the expression of interferon-γ(IFN-γ),interleukin-4(IL-4),prostaglandin F2α(PGF2α),and connexin 43(CX43).Results:There were significant differences in IFN-γ,IL-4,and PGF2α between the model group and the blank group and between the low-dose Yimu Suogong Granule group and the control group,and there was a significant difference in IL-4 between the middle-and high-dose Yimu Suogong Granule groups and the control group;there were significant differences in IFN-γ and PGF2αbetween the low-dose Yimu Suogong Granule group and the model group,and there were significant differences in IFN-γ,IL-4,and PGF2α between the middle-and high-dose Yimu Suogong Granule groups and the model group and between the control group and the model group(P＜0.01).There was a significant difference in IFN-γ/IL-4 between the model group and the blank group,between the low-,middle-,and high-dose Yimu Suogong Granule groups and the model group,between the control group and the model group,and between the low-,middle-,and high-dose Yimu Suogong Granule groups and the control group(P＜0.01).There was a significant difference in CX43 OD value between the model group and each administration group,between the middle-and high-dose Yimu Suogong Granule groups and the model group,between the control group and the model group,and between the low-dose Yimu Suogong Granule group and the control group(P＜0.01).Conclusion:Yimu Suogong Granule can cause maternal-fetal immunological rejection by regulating the shift of Th1/Th2 subsets to Th1,promote the degeneration and necrosis of residual decidual tissue and chorion tissue,and reduce intrauterine residues after incomplete abortion,possibly by promoting the contraction of uterine smooth muscle.
incomplete medical abortionYimu Suogong GranuleT-helper type 1/T-helper type 2 subsetscon-nexin 43prostaglandin 2a
万方数据
维普
