Cutaneous pharmacodynamics of Qibao Meiran tincture in treatment of alopecia
Objective:To investigate the effect of Qibao Meiran tincture on the cyclic growth of hair and hair follicles in Sprague-Dawley(SD)rats.Methods:A total of 60 SD rats were randomly divided into 10％,30％,50％,and 70％Qibao Meiran tincture groups,minoxidil group,and blank control group,with 10 rats in each group.External application of hair removal cream was performed for modeling with an area of about 40 cm2(8 cm × 5 cm),and drug intervention was performed since the day after modeling.The rats in the Qibao Meiran tincture groups were given external application of Qibao Meiran tincture at different con-centrations,while those in the minoxidil group were given external application of 5％minoxidil tincture,at a volume of 1 mL each time,twice a day for 21 consecutive days,and the drugs were rubbed to absorption;the rats in the blank control group were not given any drug intervention.The color and condition of the skin were observed and recorded.After intervention,12 sites of newly grown hair were randomly selected and were measured and recorded using a vernier caliper with percentile electronic dig-ital display;a piece of skin was collected from the observation area,and HE staining was performed to observe the thickness of the corium layer,the number of hair follicles,and morphological changes.Results:Compared with the blank control group,the Qibao Meiran tincture groups had a significant reduction in the time for the skin to change from pink to white color and signifi-cant increases in the length of hair and the number of hair follicles,and the 50％Qibao Meiran tincture group showed the shor-test time(P＜0.05).On day 21 of intervention,the 50％Qibao Meiran tincture group was comparable to the minoxidil group in terms of the length of hair and the number of hair follicles(P＞0.05),with significantly better results than the other groups(P＜0.05).Compared with the blank control group,the 30％,50％,and 70％Qibao Meiran tincture groups had a significant in-crease in the thickness of the corium layer(P＜0.01),with no significant difference compared with the minoxidil group(P＞0.05).Conclusion:Qibao Meiran tincture can accelerate hair growth in rats and promote transformation during the growing stage of hair follicles,and 50％Qibao Meiran tincture has a comparable clinical effect to minoxidil.
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