基于网络药理学和实验验证探析"虎杖-威灵仙"药对治疗痛风性关节炎的作用机制
Mechanism of action of Polygonum cuspidatum-Clematis chinensis drug combination in treatment of gouty arthritis:A study based on network pharmacology and experimental validation
李云鹤 1庞静 1李纯璞 2魏开斌 2贾玉立 1郭冬梅2
作者信息
- 1. 泰安市中医医院,山东 泰安,271000
- 2. 泰安市中心医院,山东 泰安,271000
- 折叠
摘要
目的:基于网络药理学和实验验证探析"虎杖-威灵仙"药对治疗痛风性关节炎的作用机制.方法:通过TCMSP平台筛选"虎杖-威灵仙"药对的主要活性成分及作用靶点,应用GenneCards数据库预测痛风性关节炎的相关靶点,制作韦恩图获取"虎杖-威灵仙"药对与痛风性关节炎的共同靶点,运用Cytoscape 3.7.1软件绘制中药-成分-疾病-靶点调控网络,利用STRING数据库进行蛋白质-蛋白质相互作用网络的构建与分析.最后通过R语言和Bioconductor平台进行基因本体(GO)功能和京都基因与基因组百科全书(KEGG)通路富集分析,并通过动物实验进行验证.结果:"虎杖-威灵仙"药对主要通过12种活性成分、26个作用靶点及152个生物学通路发挥治疗痛风性关节炎的作用.其中主要核心靶点为白细胞介素-1B(IL-1B)、趋化因子配体2(CCL2)、基质金属蛋白酶9(MMP9)等;关键通路为白细胞介素-17(IL-17)信号通路.经过"虎杖-威灵仙"药对干预后,小鼠关节液中IL-17的含量有降低趋势,但差异无统计学意义(P＞0.05).结论:"虎杖-威灵仙"药对具有多成分、多靶点、多通路的特点,其作用机制可能与降低IL-17水平有关.
Abstract
Objective:To investigate the mechanism of action of the Polygonum cuspidatum-Clematis chinensis drug combination in the treatment of gouty arthritis based on network pharmacology and experimental validation.Methods:The TCMSP platform was used to obtain the main active components and action targets of the Polygonum cuspidatum-Clematis chinensis drug combination,GenneCards database was used to predict the targets associated with gouty arthritis,and Venn diagram was plotted to obtain the intersecting targets between the Polygonum cuspidatum-Clematis chinensis drug combination and gouty arthritis.Cytoscape 3.7.1 was used to construct a traditional Chinese medicine-component-disease-target regulatory network,and STRING database was used to construct and analyze the protein-protein interaction network.R language and Bioconductor platform were used to perform gene ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis,and animal experiments were con-ducted for validation.Results:The Polygonum cuspidatum-Clematis chinensis drug combination exerted a therapeutic effect on gouty arthritis through 12 active components,26 action targets,and 152 biological pathways.The main targets in-cluded IL-1B,CCL2,and MMP9,and the key pathway was the IL-17 signaling pathway.After intervention with the Polyg-onum cuspidatum-Clematis chinensis drug combination,there was a tendency of reduction in the content of IL-17 in syno-vial fluid(P＞0.05).Conclusion:The Polygonum cuspidatum-Clematis chinensis drug combination exerts a therapeutic effect through multiple components,targets,and pathways,possibly by reducing the level of IL-17.
关键词
痛风性关节炎/虎杖/威灵仙/作用机制/网络药理学Key words
gouty arthritis/Polygonum cuspidatum/Clematis chinensis/mechanism of action/network pharmacology引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据