Identification of key genes and prediction of traditional Chinese medicine drugs for ischemic cardiomyopathy
Objective:To investigate the differentially expressed genes(DEGs)in ischemic cardiomyopathy(ICM)using bioinformatics techniques and machine learning algorithms,to predict the traditional Chinese medicine(TCM)drugs with an interventional effect on Hub genes,and to provide a scientific basis for further explore the mechanism of action for ICM and the medication rule of TCM drugs.Methods:Gene Expression Omnibus database was used to obtain the data on left ventricular tissue sections in ICM or non-ICM patients in the GSE116250 data-set.Limma package was used to identify the DEGs between the two groups,and Gene Set Enrichment Analysis was performed for these DEGs.The weighted gene co-expression network analysis was used to obtain the gene modules with the highest degree of association with clinical properties,which were intersected with DEGs to obtain secondary Hub genes,and gene ontology functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes path-way enrichment analysis were performed for these genes.A protein-protein interaction network was established for secondary Hub genes,and a random forest regression model and a support vector machine-recursive feature elimina-tion model were established.Hub genes were obtained,and Coremine Medical database was used to predict TCM drugs with an interventional effect on Hub genes and collect related information such as nature,taste,meridian en-try,and function.Results:A total of 805 DEGs and 8 Hub genes were obtained.The Gene Set Enrichment Analysis showed that DEGs were significantly enriched in the pathways of oxidative phosphorylation,interaction between ex-tracellular matrix and receptor,transendothelial migration of leukocytes,transforming growth factor-β signaling path-way,and Toll-like receptor signaling pathway.The prediction of TCM drugs showed that they mainly had a neutral,warm or cold nature and a bitter,sweet or pungent taste,and they mainly entered the liver,lung,and spleen meridi-ans.Tonifying drugs,heat-clearing drugs,diuresis-inducing and dampness-draining drugs,and blood-activating and stasis-resolving drugs were the main types of drugs.Conclusion:This study obtains eight Hub genes for ICM and the targeted TCM drugs for the treatment of ICM,which may have an anti-inflammatory effect,an anti-myocar-dial apoptosis effect,and an effect in regulating oxidative stress response.The above results provide a reference for the pathogenesis of ICM and prescription and medication for ICM.
ischemic cardiomyopathybioinformaticsdifferentially expressed genestraditional Chinese medicine drugs
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