Effect of Huxinkang on ferroptosis and apoptosis of H9C2 cardiomyocytes induced by hypoxia/reoxygenation
Objective:To investigate the effect of serum containing Huxinkang in inhibiting the ferroptosis and apoptosis of H9C2 cardiomyocytes induced by hypoxia/reoxygenation(H/R)by regulating the nuclear factor erythroid 2-related factor 2(Nrf2)-glutathione peroxidase 4(GPX4)pathway.Methods:H9C2 rat cardiomyocytes were used to establish a model of H/R-induced injury and were then divided into blank group,model group,12％serum containing Huxinkang group(12％Huxinkang group),6％serum containing Huxinkang group(6％Huxinkang group),and ferropto-sis inhibitor group(Fer-1 group).Cell apoptosis rate was observed,as well as the levels of malondialdehyde(MDA),fer-rous ion(Fe2+),glutathione(GSH),and N-terminal pro-brain natriuretic peptide(NT-proBNP)and the expression levels of GPX4 and Nrf2.Results:There were significant differences between the model group and the blank group in cell apoptosis rate,the levels of MDA,Fe2+,GSH,and NT-proBNP,and the protein expression levels of Nrf2 and GPX4(P＜0.05).There were significant differences between the Huxinkang groups/the Fer-1 group and the model group in cell apoptosis rate,the levels of GSH and MDA and the protein expression level of Nrf2;there were significant differences in the levels of Fe2+and NT-proBNP between the 12％Huxinkang group and the model group;there was a significant difference in the protein expression level of GPX4 between the Huxinkang groups and the model group(P＜0.05).Conclusion:Serum containing Huxinkang can alleviate ferroptosis and apoptosis of H9C2 cardiomyocytes induced by H/R,possibly by inhibiting the expression of Fe2+and MDA and upregulating the protein expression of Nrf2 and GPX4.
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