护心康对缺氧/复氧诱导的H9C2心肌细胞铁死亡和细胞凋亡的影响
Effect of Huxinkang on ferroptosis and apoptosis of H9C2 cardiomyocytes induced by hypoxia/reoxygenation
何霞 1常嘉昱 1朱筱婧 2张婷 2庄红 2卿俊 1陈志红 2蒋岱君 1颜旭2
作者信息
- 1. 湖南中医药大学,湖南 长沙,410208
- 2. 湖南省中西医结合医院,湖南 长沙,410006
- 折叠
摘要
目的:探究护心康含药血清通过调控核因子-E2-相关因子2-谷胱甘肽过氧化物酶4(Nrf2-GPX4)通路对抑制缺氧/复氧(H/R)大鼠H9C2心肌细胞铁死亡和细胞凋亡的影响.方法:构建H/R诱导的大鼠心肌细胞H9C2损伤模型,分为空白组、模型组、12％护心康含药血清组(12％护心康组)、6％护心康含药血清组(6％护心康组)及铁死亡抑制剂组(Fer-1组).观察细胞凋亡率,丙二醛(MDA)、亚铁离子(Fe2+)、谷胱甘肽(GSH)、N-末端B型利钠肽原(NT-proBNP)水平,谷胱甘肽过氧化物酶4(GPX4)和核因子-E2-相关因子2(Nrf2)的表达.结果:模型组细胞凋亡率,MDA、Fe2+、GSH、NT-proBNP水平,Nrf2、GPX4蛋白表达水平,与空白组比较,差异均有统计学意义(P＜0.05);护心康各含药血清组及Fer-1组细胞凋亡率及GSH、MDA、Nrf2蛋白表达水平,12％护心康组Fe2+、NT-proBNP水平,护心康各含药血清组GPX4蛋白表达水平,与模型组比较,差异均有统计学意义(P＜0.05).结论:护心康含药血清能减轻H/R诱导的H9C2心肌细胞铁死亡和凋亡,其机制可能与抑制Fe2+、MDA表达,上调Nrf2和GPX4蛋白表达有关.
Abstract
Objective:To investigate the effect of serum containing Huxinkang in inhibiting the ferroptosis and apoptosis of H9C2 cardiomyocytes induced by hypoxia/reoxygenation(H/R)by regulating the nuclear factor erythroid 2-related factor 2(Nrf2)-glutathione peroxidase 4(GPX4)pathway.Methods:H9C2 rat cardiomyocytes were used to establish a model of H/R-induced injury and were then divided into blank group,model group,12％serum containing Huxinkang group(12％Huxinkang group),6％serum containing Huxinkang group(6％Huxinkang group),and ferropto-sis inhibitor group(Fer-1 group).Cell apoptosis rate was observed,as well as the levels of malondialdehyde(MDA),fer-rous ion(Fe2+),glutathione(GSH),and N-terminal pro-brain natriuretic peptide(NT-proBNP)and the expression levels of GPX4 and Nrf2.Results:There were significant differences between the model group and the blank group in cell apoptosis rate,the levels of MDA,Fe2+,GSH,and NT-proBNP,and the protein expression levels of Nrf2 and GPX4(P＜0.05).There were significant differences between the Huxinkang groups/the Fer-1 group and the model group in cell apoptosis rate,the levels of GSH and MDA and the protein expression level of Nrf2;there were significant differences in the levels of Fe2+and NT-proBNP between the 12％Huxinkang group and the model group;there was a significant difference in the protein expression level of GPX4 between the Huxinkang groups and the model group(P＜0.05).Conclusion:Serum containing Huxinkang can alleviate ferroptosis and apoptosis of H9C2 cardiomyocytes induced by H/R,possibly by inhibiting the expression of Fe2+and MDA and upregulating the protein expression of Nrf2 and GPX4.
关键词
护心康/含药血清/缺氧/复氧损伤/H9C2心肌细胞/铁死亡/细胞凋亡Key words
Huxinkang/serum containing/hypoxia/reoxygenation injury/H9C2 cardiomyocytes/ferroptosis/cell apoptosis引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据