Mechanism of action of Dongshen Pill in treatment of renal interstitial fibrosis:A study based on network pharmacology and molecular docking
Objective:To investigate the mechanism of action of Dongshen Pill in the treatment of renal interstitial fibrosis based on network pharmacology and molecular docking.Methods:TCMSP and HERB were used to obtain the active components of Dongshen Pill,and SwissTargetPrediction was used to predict the action targets of active components.GeneCards,OMIM,and DisGeNET were used to obtain disease targets,and Venny 2.0 was used to obtain the intersecting targets of the drug and the disease.STRING database was used to construct a protein-protein interaction net-work for the intersecting targets.Cytoscape 3.10.0 was sued to identify core targets,and DAVID database was used to per-form the gene ontology(GO)functional enrichment analysis and the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis of the core targets.Results:A total of 250 active components and 676 action targets were ob-tained for the drug Dongshen Pill,and 2069 targets were obtained for the disease renal interstitial fibrosis,with 240 inter-secting targets and 43 core targets.The main active components included baicalin,4',5-dihydroxyflavone,luteolinidin,quercetin,magnoloside B,and kadsurenone,and the core targets included AKT1,EGFR,STAT3,TNF,CASP3,JUN,BCL2,and MAPK3.The GO functions mainly involved cell apoptosis,inflammation,and oxidative phosphorylation,and the KEGG pathways mainly involved nuclear factor-kappa B,calcium channel,and cGMP-PKG.Conclusion:Dongshen Pill exerts the pharmacological actions such as anti-inflammation,antioxidation,regulation of cell apoptosis,inhibition of epi-thelial-mesenchymal transition,and lipid metabolism through the targets including AKT1,EGFR,and STAT3,thereby im-proving renal fibrosis.
renal interstitial fibrosisDongshen Pillmechanism of actionnetwork pharmacologymolecular docking
NETL
NSTL
万方数据
