Analysis of pathogenic characteristics of cholera caused by non-O1/O139 Vibrio cholera
Objective To understand the gene sequence and drug resistance characteristics of non-O1/O139 group V.cholera,and provide information for cholera genomics,so as to provide reference for handling emergency.Methods Epidemiological investigation was carried out among patients and the feces samples were collected for isolation and culture.Biochemical tests and matrix-assisted laser desorption ionization time-of-flight mass spectrometry(Bruker MALDI Biotype sirius one)were used for identification,and slide agglutination was used for serogroup/type identification.The drug susceptibility test was carried out with microbroth dilution method.The Illumina Hiseq™ sequencing platform was used for bacterial 16S rRNA gene amplification and whole genome sequencing.Virulence gene and drug resistance gene were detected and analyzed.Results The patient drank un-boiled water.There was no patient found in family members,neighbors(who dined at the patient's home),and 37 people from 10 households on the 5th floor of the same unit.In the patient's feces samples,non-O1/O139 V.cholera was detected with pathogenic gene and resistance to polymyxin E.The strain was identified as ST841,a new type of strain by PubMLST typing database.The sequence of the closest edge obtained by comparison was V.cholerae reference strain ERR1024532/1995/Latin America/non O1.The difference between the number of nuclear genes and the nearest genome sequences was 328.Conclusions The pathogen of this patient is non-O1/O139 V.cholera.The monitoring of food safety of V.cholera and foodborne disease surveillance and report should be strengthened for outbreak prevention of foodborne diseases.
Non-O1/O139 group V.choleraeDrug susceptibility testVirulence geneWhole genome sequencing
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