摘要
目的 基于16SrRNA高通量测序技术探讨新疆不同地域、不同年龄儿童肠道菌属构成.方法 收集2023年4月-2023年11月乌鲁木齐市儿童医院门/急诊及住院儿童粪便样本194份,提取DNA并测序,开展聚类、多样性分析,根据测序结果绘制新疆地区儿童的肠道菌群谱,从而分析不同地域、不同年龄儿童肠道菌群之间的差异性.结果 通过16S rRNA高通量测序得到15 886 809条有效序列、91 736个操作性分类单元(operational taxonomic units,OTUs);儿童肠道微生物群落在门水平上的构成大小依次为壁厚菌门、放线菌门、变形菌门、拟杆菌门、疣微菌门和梭杆菌门,在属水平上的构成大小依次为双歧杆菌属、埃希杆菌属、拟杆菌属、肠球菌属、链球菌属和阿克曼菌属.南北疆Shannon(t=0.867)、SimPson(Z=0.878)、Ace(Z=-0.740)和 Chao(Z=-0.566)指数差异均无统计学意义(P＞0.05);3 个年龄组Shannon(F=16.627)、Ace(H=24.300)、Chaol(H=28.570)和 SimPson(H=18.323)指数差异均有统计学意义(P＜0.05).结论 新疆不同地域、不同年龄组儿童的肠道菌群存在差异,儿童肠道微生物呈多样性.
Abstract
Objective To analyze the composition of intestinal bacterial genera in children as different areas and different ages in Xinjiang,based on 16S rRNA high-throughput sequencing technology.Methods A total of 194 fecal samples were collected from children patients in the department of outpatient/emergency and inpatient in Urumqi Children*s Hospital from April to November 2023.The DNA of pathogen was extracted and sequenced.The cluster and diversity analysis were carried out,and the intestinal flora spectrum was plotted in the children of Xinjiang for analyzing the differences of intestinal bacterial genera of children in different areas and different ages.Results There were 15 886 809 valid sequences and 91 736 operational taxonomic units(OTUs)were obtained by 16S rRNA high-throughput sequencing.The composition of intestinal microbiota in children was in the order of Firmicutes,Actinobacteria,Proteobacteria,Bacteroidetes,Verrucomicrobia,and Fusobacteria at the phylum level,and Bifidobacterium,Escherichia,Bacteroides,Enterococcus,Streptococcus,and Akkermansia at the genus level.There were no statistical differences between the indexes of Shannon(t=0.867),Simpson(Z=0.878),Ace(Z=-0.740)and Chao(Z=-0.566)in northern and southern Xinjiang(P＞0.05).There were statistical differences(P＜0.05)in the indexes of Shannon(F=16.627),Ace(H=24.300),Chaol(H=28.570),and SimPson(H=18.323)in three age groups.Conclusions There are differences in intestinal bacterial genera among children in different areas and age groups in Xinjiang.The intestinal microbiota show diversity in children.
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