基于单细胞尺度的纳米移液管递送研究
Precision Delivery Using Nanopipette for Single-cell Studies
张鹤 1Md Maksudur Rahman 1陶洋 1Joseph W Sampson 1任航2
作者信息
- 1. Department of Chemistry,The University of Texas at Austin,Austin,TX 78712,USA
- 2. Department of Chemistry,The University of Texas at Austin,Austin,TX 78712,USA;Center for Electrochemistry,The University of Texas at Austin,Austin,TX 78712,USA;Texas Materials Institute,The University of Texas at Austin,Austin,TX 78712,USA
- 折叠
摘要
基于纳米移液管的电化学扫描探针技术是一种多功能非接触成像工具,并被广泛应用于生物学研究.除了一般的表面形貌成像研究外,它在局部递送生物活性分子方面的潜力也逐渐显现.在这篇简短综述中,我们介绍了这种技术在单细胞研究中的应用,特别是局部递送.我们总结并比较了三种递送模式的工作原理,包括阻抗脉冲、压力驱动和电渗流驱动递送,还回顾了这些模式在单细胞研究中的应用.此外,本文还讨论了基于扫描离子电导显微镜的递送技术所面临的技术挑战及其在医学和药理学研究中日益增长的影响力.
Abstract
Nanopipette based scanning probe technique is a versatile tool in non-contact imaging in biology.In addition to the topographic imaging,its capability of localized delivery of bio-active molecules is emerging.In this mini review,we introduce the applications of nanopipette in single-cell researches with a focus on localized delivery.The working principles of three delivery modes including resistive pulse,pressure-driven flow,and electroosmotic flow-driven delivery are summarized and compared.Their applications in single-cell researches are reviewed.The current technical challenges in scanning ion conductance microscopy-based delivery,and their growing influence in medicine and pharmacologic researches are also discussed.
关键词
扫描离子电导显微镜/纳米移液管/单细胞成像/局部递送Key words
Scanning ion conductance microscopy/Nanopipette/Single cell image/Local delivery引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据