基于生物三维打印的体外药筛模型构建
Construction of in Vitro Drug Screening Models Based on Bio-3D Printing
胡晨钰君 1盖可 2徐才哲 2周玉杰 3张磊4
作者信息
- 1. 北京市心肺血管疾病研究所,北京 100011
- 2. 清华大学机械工程系生物制造中心,北京 100084
- 3. 北京市心肺血管疾病研究所,北京 100011;首都医科大学附属北京安贞医院老年心血管病中心,北京 100011
- 4. 清华大学机械工程系生物制造中心,北京 100084;生物制造与快速成形技术北京市重点实验室,北京 100084
- 折叠
摘要
以生物三维打印技术为基础,构建了一种体外三维模型药物筛选模型,并采用双向温控喷头挤出式的打印方式完成模型打印,将构成血脑屏障的两种细胞与天然水凝胶混合后层层打印,从而得到体外药筛模型.通过检测模型的弹性模量、微观结构及细胞特异性蛋白的表达,在功能上与空白对照组及二维细胞组进行对比.结果表明,相较于空白组及二维细胞培养组,打印完成的体外药筛模型弹性模量稳定,内部形成适合细胞生长的多孔结构,证明具有较好的生物相容性与结构稳定性,对于胰岛素分子的筛选中有着显著优势.
Abstract
Based on biological 3D printing,an in vitro 3D model drug screening model was constructed,and the model printing was completed by using a bi-directional temperature controlled extrusion-based printing method.Two types of cells that constitute the blood-brain barrier were mixed with natural hydrogel and printed layer by layer to obtain the in vitro drug screening model.By detecting the elastic modulus,microstructure and expression of cell specific proteins of the model,functional comparisons were made with a two-dimensional cell group.The result showed that compared to the blank group and two-dimensional cell group,the printed in vitro drug screening model had a stable elastic modulus and formed a porous structure suitable for cell growth,which indicating good biocompatibility and structural stability and significant advantage in screening insulin molecules.
关键词
生物三维打印/血脑屏障/药筛模型/渗透性Key words
biological 3D printing/blood-brain barrier/drug screening model/permeability引用本文复制引用
基金项目
国家自然科学基金项目(52375295)
高等学校学科创新引智计划(111计划)项目(B17026)
出版年
2024
NETL
NSTL
万方数据