Construction of in Vitro Drug Screening Models Based on Bio-3D Printing
Based on biological 3D printing,an in vitro 3D model drug screening model was constructed,and the model printing was completed by using a bi-directional temperature controlled extrusion-based printing method.Two types of cells that constitute the blood-brain barrier were mixed with natural hydrogel and printed layer by layer to obtain the in vitro drug screening model.By detecting the elastic modulus,microstructure and expression of cell specific proteins of the model,functional comparisons were made with a two-dimensional cell group.The result showed that compared to the blank group and two-dimensional cell group,the printed in vitro drug screening model had a stable elastic modulus and formed a porous structure suitable for cell growth,which indicating good biocompatibility and structural stability and significant advantage in screening insulin molecules.
biological 3D printingblood-brain barrierdrug screening modelpermeability
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