Study of miR-483-3p increasing sensitivity of pancreatic cancer to gemcitabine and molecular mechanism
Gemcitabine resistance limits its therapeutic effect to pancreatic cancer,and traditional chemical drugs cannot effectively overcome gemcitabine resistance to pancreatic cancer.Studies have shown that abnormal expression of microRNA(miRNA)is associated with tumorigenesis,development and chemoresistance.The role and potential mechanism of miR-483-3p in gemcitabine resistance to pancreatic cancer are investigated.qPCR results show that miR-483-3p is significantly down-regulated in pancreatic cancer drug-resistant cells.Transient transfection of miR-483-3p minics significantly increases the expression level of miR-483-3p in drug-resistant cells,significantly weakens the proliferation,migration,metastasis and invasion ability of drug-resistant cells,and restores the reactivity of gemcitabine.Western blot results show that miR-483-3p inhibits the expression levels of P13K and p-AKT proteins,inhibits the expression of Bc12 protein,promotes the expression of Bax,Bad and cleaved-Caspase-3 proteins,and promotes the process of apoptosis.In conclusion,the results suggest that miR-483-3p promotes apoptosis by inhibiting the PI3K/AKT signaling pathway and increases the sensitivity of pancreatic cancer to gemcitabine,and miR-483-3p may be a potential therapeutic strategy for gemcitabine-resistant pancreatic cancer patients.
万方数据
维普
