Extraction of soybean saponin Ⅱ in soybean seeds and its analysis by HPLC-MS
Soybean saponin has been shown as compound with physiological activities and pharmacological action, the bioactivities of which are related to its structure. The extraction, separation and determination of group B of soybean saponin was studied in this paper. The soybean saponin was extracted from soybean seeds with AB-8 resin column, and separated through silica column chromatography. In the extraction and separation of group B of soybean saponnins, HPLC-MS was employed to determine soybean saponin Ⅱ. The method was convenient and useful.
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