The mimicking human fat globule structure emulsion and its applications in vitro digestion
Himlar7500 whey protein powder rich in fat globular membrane(MFGM)was used to prepare a model oil in water lotion with a structure similar to that of breast milk fat globules using soybean oil.The oxidation stability and digestion difference of infant formula,breast milk and prepared lotion were compared in storage experiment and simulated in vitro digestion experiment.Compared the similarities between the particle size distribution and that of breast milk,it was found that the particle size distribution of the oil-in-water lotion prepared by 4%MFGM,shear rate of 12 000 r/min,homogenization pressure of 35 MPa and 40%soybean oil showed a bimodal distribution,which was similar to that of breast milk.In the storage experiment at 60 ℃,the initial potential of the prepared lotion was-39.36 mV and remained-35.60 mV after 12 d,with less change than that of breast milk and infant formula.Within 12 d,the particle size,POV content and TBARS content changed slightly,which proved that the lotion was with good oxidation stability.In the digestion experiment,the fatty acid releasing rate of lotion was 61.53%,which was lower than that of breast milk(78.56%),but had no significant differences between that of milk powder(66.09%).It showed a similar fatty acid release pattern with that of breast milk,which proved that the model lotion had good structural similarity with breast milk.