首页|拮抗菌Variovorax sp.R1对南果梨糖代谢的影响

拮抗菌Variovorax sp.R1对南果梨糖代谢的影响

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机械损伤会导致南果梨果实的呼吸速率大幅增加,加速果实的衰老腐败。本实验使用拮抗菌Variovorax sp。R1处理南果梨,研究了拮抗菌对机械损伤胁迫下南果梨糖代谢的影响。结果表明,R1发酵上清液和菌悬液处理组果实的蔗糖含量都增加了,5 d时分别是对照组的1。71倍和1。12倍。两个R1处理组的蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)活性都提高了。R1发酵上清液处理组果实的葡萄糖、果糖含量和山梨醇脱氢酶(SDH)活性也提高了,7 d时分别比对照组提高7。06%、18。52%、4。22%。相关性分析结果表明,蔗糖含量与SS、SPS活性呈显著正相关;葡萄糖、果糖含量与SDH活性呈显著正相关。结果显示,拮抗菌R1通过调节糖代谢相关酶的活性,可以提高南果梨的可溶性糖含量,有助于维持机械损伤胁迫下南果梨的品质。
Effect of the antagonistic bacterium Variovorax sp.R1 on the sugar metabolism of Nanguo pears
A significant increasing in the respiratory rate of the fruit can be caused by mechanical damage,accelerating its aging and decay.The antagonistic bacterium Variovorax sp.R1 was used to treat Nanguo pear and the effect of antagonistic bacteria on sugar metabolism in Nanguo pear under mechanical damage stress was studied.The result showed the sucrose contents in the fruits of the R1 fermentation supernatant and bacterial suspension treatment groups increased,which was 1.71 and 1.12 times higher than that of the control group after 5 d,respectively.The activities of sucrose synthase(SS)and sucrose phosphate synthase(SPS)were both increased in R1 treatment groups.The glucose and fructose contents and sorbitol dehydrogenase(SDH)activity of the fruits in the R1 fermentation supernatant treatment group were also increased,with an increase of 7.06%,18.52%and 4.22%respectively,compared to the control group after 7 d.The correlation analysis results showed that sucrose content was significantly positively correlated with SS and SPS activities,while the contents of glucose and fructose were significantly positively correlated with SDH activity.The results indicated that the soluble sugar content of Nanguo pear could be increased by antagonistic bacterium R1 through regulating the activity of sugar metabolism related enzymes,contributing to maintain the quality of Nanguo pear under mechanical damage stress.

antagonistic endophytic bacteriaNanguo pearmechanical damagesucrose metabolisms

杨碧莹、王璐、叶淑红、王悦潞

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大连工业大学食品学院,辽宁 大连 116034

拮抗内生菌 南果梨 机械损伤 蔗糖代谢

国家自然科学基金辽宁省自然科学基金

32171836LJKZ0538

2024

大连工业大学学报
大连工业大学

大连工业大学学报

影响因子:0.295
ISSN:1674-1404
年,卷(期):2024.43(2)
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