摘要
利用静电喷雾技术开发了一种可口服的葛根素微球——以海藻酸盐为载体负载葛根素的载药凝胶微球.光学显微镜及扫描电镜显示葛根素微球的球形度高,分散性好,平均粒径(391.62±22.06)μm.体外释放曲线表明葛根素微球能够避免胃酸分解,到达肠道缓慢释放,从而提高葛根素的生物利用率.将葛根素微球给予小鼠10周后,与模型组相比,葛根素微球组的小鼠血糖下降了约31.57％,肝脏指数下降了18.67％.Masson染色结果显示,葛根素微球能显著降低肝脏组织纤维化程度,改善肝功能.Western Blot检测各组小鼠肝脏中糖原合成相关蛋白GSK-3β和GYS-2表达,与模型组相比,葛根素微球组GSK-3β表达下降了69.33％,GYS-2表达升高了126.19％.结果表明,通过口服葛根素微球能够有效促进肝糖原的合成,降低血糖,从而减轻2型糖尿病症状及其引起的肝损伤.
Abstract
An oral puerarin microsphere(alginate supported puerarin drug-carrying gel microsphere)was developed by electrostatic spray technology.Optical microscopy and scanning electron microscopy results showed that the sphericity and dispersibility of puerarin microspheres were high,with an average particle size of(391.62±22.06)μm.The release curve in vitro showed that puerarin microspheres could avoid gastric acid decomposition and release to the intestine slowly,thereby increasing the bioavailability of puerarin.After 10 weeks of administration of puerarin microspheres to mice,the blood glucose levels in the puerarin microspheres group was decreased by approximately 31.57％and the liver index was decreased by 18.67％compared with the model group.Masson staining result showed that the degree of liver fibrosis could be effectively reduced by puerarin microspheres and liver function are improved.The expression of glycogen synthesis related proteins GSK-3β and GYS-2 in mice liver in each group were detected by Western Blot.Compared with the model group,the expression of GSK-3β in the puerarin microsphere group was decreased by 69.33％,while the expression of GYS was increased by 126.19％.The results showed that oral administration of puerarin microspheres could effectively promote the synthesis of liver glycogen,reduce blood sugar,and thus alleviate the symptoms of type 2 diabetes and the liver injury caused by type 2 diabetes.
国家科技期刊平台
NSTL
维普
万方数据