Preparation of puerarin microspheres and their protective effect on liver injury in type 2 diabetes mellitus
An oral puerarin microsphere(alginate supported puerarin drug-carrying gel microsphere)was developed by electrostatic spray technology.Optical microscopy and scanning electron microscopy results showed that the sphericity and dispersibility of puerarin microspheres were high,with an average particle size of(391.62±22.06)μm.The release curve in vitro showed that puerarin microspheres could avoid gastric acid decomposition and release to the intestine slowly,thereby increasing the bioavailability of puerarin.After 10 weeks of administration of puerarin microspheres to mice,the blood glucose levels in the puerarin microspheres group was decreased by approximately 31.57％and the liver index was decreased by 18.67％compared with the model group.Masson staining result showed that the degree of liver fibrosis could be effectively reduced by puerarin microspheres and liver function are improved.The expression of glycogen synthesis related proteins GSK-3β and GYS-2 in mice liver in each group were detected by Western Blot.Compared with the model group,the expression of GSK-3β in the puerarin microsphere group was decreased by 69.33％,while the expression of GYS was increased by 126.19％.The results showed that oral administration of puerarin microspheres could effectively promote the synthesis of liver glycogen,reduce blood sugar,and thus alleviate the symptoms of type 2 diabetes and the liver injury caused by type 2 diabetes.
国家科技期刊平台
NSTL
万方数据
维普
