Screening of Lactiplantibacillus plantarum inhibiting Fusarium graminearum in barley and its application in malting
There are various microorganisms on the surface of barley,among which Fusarium graminearum and its secreted mycotoxins can affect the malting process and malt quality.To eliminate the adverse effects of F.graminearum,a strain with significant inhibitory effect was isolated and screened from the surface of barley using MRS selective culture medium.Based on morphological observation,physiological and biochemical experiments,and 16S rDNA gene sequence analysis,the strain was identified as Lactiplantibacillus plantarum named BY66.During the wheat making process,the biomass and deoxynivalenol content of F.graminearum could be reduced by 56.2％and 79.1％,respectively,through adding 0.5 mL/kg of BY66 fermentation broth.The negative effects of F.graminearum on barley germination and malt quality also could be eliminated by adding BY66 fermentation broth.Compared with the infected barley,the germination rate of barley could be increased by 31％with the addition of 0.5 mL/kg BY66 fermentation broth.
国家科技期刊平台
NSTL
万方数据
维普
