大豆分离蛋白制备南极磷虾油水包油乳液的作用
Effect of soybean protein isolate on the preparation of Antarctic krill oil-in-water emulsion
吕璐 1王紫阳 1刘玉佳1
作者信息
- 1. 大连工业大学 生物工程学院,辽宁 大连 116034
- 折叠
摘要
以不同质量浓度大豆分离蛋白(SPI)为乳化剂,利用高速均质和超声波破碎两步法制备了SPI-南极磷虾油水包油乳液,研究了大豆分离蛋白作为乳化剂的作用.结果表明,当SPI质量浓度从2 mg/mL增加到16 mg/mL时,蛋白溶液的界面张力从50.1 mN/m减小到42.8 mN/m,乳化稳定性从95.3％增加到98.4％,发泡稳定性从70.8％显著降低到41％,粒径范围127.3～146.3 nm,界面蛋白吸附量从47％增加到74％,共聚焦和表观观察发现所有样品分布均匀且稳定.在储存期间,吐温20乳液过氧化值从初始的0.555 mmol/kg上升到1.938 mmol/kg,蛋白质量浓度4 mg/mL的SPI乳液过氧化值从初始的0.665 mmol/kg上升到1.170 mmol/kg,表明SPI具有抑制氧化作用的能力.蛋白质量浓度为4 mg/mL的SPI乳液在40、50、60 ℃的货架期分别为31.4、30.2和29.2 d.
Abstract
The soy protein isolate(SP1)oil-in-water lotion of Antarctic krill was prepared by high-speed homogenization and ultrasonic crushing with different concentrations of SPI as emulsifier.The effect of SPI as emulsifier was studied.The results showed that when the SPI concentration increased from 2 to 16 mg/mL,the interfacial tension of the protein solution decreased from 50.1 to 42.8 mN/m,while the emulsification stability increased from 95.3％to 98.4％,and the foaming stability significantly decreased from 70.8％to 41％.The particle size range was from 127.3 to 146.3 nm,and the interfacial protein adsorption increased from 47％to 74％.Confocal and epigenetic results showed that all samples were evenly distributed and stable.During storage,the peroxide value of Tween 20 lotion increased from 0.555 to 1.935 mmol/kg,while SPI lotion and Tween 20 lotion with 4 mg/mL protein increased from 0.665 to 1.170 mmol/kg.The shelf life of SPI lotion with 4 mg/mL protein at 40,50 and 60 ℃ were 31.4,30.2 and 29.2 d,respectively.
关键词
大豆分离蛋白/南极磷虾油/水包油乳液Key words
soybean protein isolate(SPI)/Antarctic krill oil/oil-in-water emulsion引用本文复制引用
基金项目
辽宁省教育厅科学研究经费项目(面上项目)(LJKZ0522)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据