Physical and chemical properties of Apostichopus japonicus catalase
A recombinant catalase(rAj-CAT)with antioxidant activity was expressed by the recombinant engineering bacterium BL21(DE3)/pET-28c-Aj-CAT.The recombinant protein rAj-CAT exhibited a characteristic peak spectrum at 405 nm,which was not affected by Na2S2O4.Typical catalase inhibitors such as 3-amino-1,2,4-triazole,hydroxylamine hydrochloride and sodium azide have significant inhibitory effects on rAj-CAT.The results indicated that the catalase was a typical single functional catalase.The optimal enzyme activity conditions for rAj-CAT were tested.The result showed that rAj-CAT had the ability to degrade H2O2 in a lower temperature range(4-30 ℃),with an optimal enzyme activity temperature of 10 ℃.The rAj-CAT has a wide pH tolerance range and exhibits catalytic activity from pH 4.0 to 11.0.The rAj-CAT can also tolerate various metal ions and chemicals,including Triton X-100,Tween 20 and EDTA.But DTT,β-ME,SDS,chymotrypsin and trypsin had strong destructive effects on the activity of rAj-CAT to hydrolyze H2O2.The results indicated that Aj-CAT of Apostichopus japonicus is a catalase with high activity at lower temperatures and stable enzymatic properties.
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