Using differential transcriptome to located functional gene of Paraburkholderia caffeinilytica CF1
An efficient caffeine-degrading strain Paraburkholderia caffeinilytica CF1 isolated from a tea garden in China.To identify genes involved in caffeine degradation,a global transcription analysis of strain CF1 growing with and without caffeine addition were carried out and differential transcriptome analysis under the two conditions were conducted.The results showed that 4 871 Unigene sequences were obtained and 96.96％of the obtained Unigene sequences could be annotated according to the whole genome sequence of strain CF1.The difference gene analysis between groups reveals that 123 genes were up-regulated and 2 669 genes were down-regulated with caffeine addition.Among the up-regulated genes,112 genes were encoded on the chromosome and 11 genes arranged side by side were encoded on the giant plasmid.By analyzing the sequence alignment and activities of key enzymes,the redundant gene cluster containing the 11 up-regulated genes of caffeine degradation was elucidated.
国家科技期刊平台
NSTL
万方数据
