Preparation and antitumor activity of polydopamine-modified multidrug nanocrystals
Polydopamine modified paclitaxel/camptothecin/astaxanthin multi-drug nanocrystals(MDNC/PDA)were prepared,and their physicochemical properties,drug release behavior,and in vitro anti-tumor activity were studied.MDNC/PDA was prepared by ultrasound enhanced anti solvent coprecipitation method,and its physicochemical properties and drug release behavior were characterized by DLS,SEM and FT-IR.Its anti-tumor activity and proapoptotic effect in vitro were preliminarily investigated by cell uptake,MTT and Annexin V-FITC/PI double staining methods.The results showed that MDNC/PDA exhibited a rough surface with a nanoscale rod structure,average particle size of(291.74±4.69)nm,PDI of 0.286±0.004,and Zeta potential of-(31.85±1.76)mV,exhibiting good stability.The drug loading of paclitaxel,camptothecin and astaxanthin were 34.6％,32.9％and 33.5％,respectively.Compared with single drug nanocrystals,MDNC/PDA were more easily taken up by MDA-MB-231 cells and had stronger anti-tumor activity(IC50=4.13 μg/mL)and proapoptotic effect in vitro.
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