The MAPK member CrMPK3 involved in the regulation of secondary metabolism in Catharanthus roseus
A member of MAPK family in Catharanthus roseus,gene CrMPK3,was selected for further investigation of its involvement in TIAs biosynthesis.Bioinformatics analysis result shows that CrMPK3 is hydrophilic,and most likely localized in cytoskeleton.Phylogenetic analysis result indicates that CrMPK3 is highly homologous to AtMPK3 in Arabidopsis thaliana.TRV-mediated VIGS technique was conducted for transient silence of the expression of gene CrMPK3,and real-time qPCR result showed that the expression levels of key enzyme genes in TIAs biosynthetic pathway,gene CrSTR,CrG10H and CrTDC,were remarkably down-regulated.The contents of vindoline and catharanthine from leaves of silenced plants were also detected reduced significantly.These results indicate that gene CrMPK3 is involved in the regulation of TIAs biosynthesis of Catharanthus roseus,which provide a theoretical significance for in-depth analysis of the biological function of plant MAPK and improve the production of natural products through genetic engineering technology in Catharanthus roseus.
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维普
