首页|Proteomics techniques in protein biomarker discovery

Proteomics techniques in protein biomarker discovery

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Protein biomarkers represent specific biological activities and processes,so they have had a critical role in cancer diagnosis and medical care for more than 50 years.With the recent improvement in proteomics technologies,thousands of protein biomarker candidates have been developed for diverse disease states.Studies have used different types of samples for proteomics diagnosis.Samples were pretreated with appropriate techniques to increase the selectivity and sensitivity of the downstream analysis and purified to remove the contaminants.The purified samples were analyzed by several principal proteomics techniques to identify the specific protein.In this study,recent improvements in protein biomarker discovery,verification,and vali-dation are investigated.Furthermore,the advantages,and disadvantages of conventional techniques,are discussed.Studies have used mass spectros-copy(MS)as a critical technique in the identification and quantification of candidate biomarkers.Nevertheless,after protein biomarker discovery,verification and validation have been required to reduce the false-positive rate where there have been higher number of samples.Multiple reaction moni-toring(MRM),parallel reaction monitoring(PRM),and selected reaction monitoring(SRM),in combination with stable isotope-labeled internal stan-dards,have been examined as options for biomarker verification,and enzyme-linked immunosorbent assay(ELISA)for validation.

biomarker discoverycancer biomarkergel-based methodsgel-free methodsmass spectroscopyproteomics

Mahsa Babaei、Soheila Kashanian、Huang-Teck Lee、Frances Harding

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Department of Biology,Faculty of Science,Razi University,Kermanshah,Iran

Faculty of Chemistry,Sensor and Biosensor Research Center(SBRC),Razi University,Kermanshah,Iran

Nanobiotechnology Department,Faculty of Innovative Science and Technology,Razi University,Kermanshah,Iran

Centre for Marine Bioproducts Development,Flinders University,Adelaide,South Australia,Australia

Monash Institute of Pharmaceutical Sciences,Monash University,Melbourne,New South Wales,Australia

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2024

10.1002/qub2.35

定量生物学(英文版)

定量生物学(英文版)

ISSN：
年,卷(期)：2024.12(1)
  • 147