首页|Integrated photothermal microcarriers for precise exosome-secreted microRNA profiling in breast cancer diagnosis

Integrated photothermal microcarriers for precise exosome-secreted microRNA profiling in breast cancer diagnosis

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Breast cancer constitutes a significant global health burden,while conven-tional diagnosis approaches may lack precision and can be discomforting for patients.Exosomes have emerged as promising biomarkers for breast cancer due to their participation in diverse pathological processes,and a convenient analysis platform is believed to greatly promote its application.In this study,we propose a novel digital PCR approach utilizing near-infrared(NIR)photo-responsive thermosensitive microcarriers integrated with black phosphorus for quantifying microRNA(miRNA)biomarkers within exo-somes.Petal-like biomimetic nanomaterials were firstly assembled for non-specific exosome capture based on the affinity effect of avidin and biotin.Photothermal-responsive microcarriers,fabricated using gelatin-based substrates blended with photothermal nanocomposite,exhibited NIR-induced heating and reversible phase transition properties.We optimized synthesis parameters on thermal response and established a programma-ble and controllable NIR light source module.The results indicated a sig-nificant elevation in the levels of biomarkers miRNA-1246 and miRNA-122,with fold increases ranging from 6.2 to 23.6 and 5.9 to 13.0,respectively,in breast cancer cell lines MCF-7 and MDA-MB-231 compared to healthy control cells HUVEC.This study offers broad prospects for utilizing exo-somes to resolve predictive biomarkers.

breast cancerdigital PCRexosomesMicroRNAphotothermal

Yunjie Shi、Yun Cheng、Peiyu Chen、Lexiang Zhang、Fangfu Ye

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School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,China

Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine,Vision and Brain Health),Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou,China

Department of Pediatrics,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou,China

Joint Centre of Translational Medicine,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China

Beijing National Laboratory for Condensed Matter Physics,Institute of Physics,Chinese Academy of Sciences,Beijing,China

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2024

10.1002/qub2.58

定量生物学(英文版)

定量生物学(英文版)

ISSN：
年,卷(期)：2024.12(4)