Objective: To develop the assay method by RP-HPLC for ferulic acid methylester nitric oxide donor (FMND) and determine the pharmacokinetics parameters in mice after oral administration. Methods: The sample was extracted by acetoacetate, followed by vortex and high speed centrifugation, dried by nitrogen flow and dissolved by mobile phase before HPLC analysis. The HPLC condition was applied with mobile phase at methanol: water (70∶30), flow rate at 1.0 mL/min, column temperature at 30 ℃. Results: FMND was absorbed quickly with a lag time of 6.6 min after oral administration in mice at the dose of 50 mg/kg, and arrived to the peak concentration at about 1.5 h. The distribution in vivo matched two-compartment model with a lag time. The distribute half life time(t1/2α)was 1.04 h, the eliminate half life time(t1/2β)was 9.44 h, the absorb half life time(t1/2 Ka)was 0.94 h. Conclusion: FMND was absorbed quickly in mice after oral administration, and the distribute equilibrium arrived moderately with a long eliminate half life time.
NETL
NSTL
维普
万方数据
