Regulation of Vernalization and Photoperiod on Heading Date in Barley
Hulless barley is a major crop on the Qinghai-Xizang Plateau in China,and its adaptability determines the planting range and yield of varieties.The heading date is an important agronomic trait affecting the adaptability of barley varieties,and timely heading and flowering is a prerequisite for high yield and wide adaptation of barley.In the vernalization pathway,HvVRN1,HvVRN2 and HvVRN3 are the major genes that determine the growth habit of barley,and in the photoperiodic pathway,HvPPD-H1 and HvPPD-H2 are important genes that control the heading stage of barley.In order to investigate the regulation mechanism of vernalization and photoperiod on the heading,this study used Dongqing18(D18)and Chengwumang(K18)as experimental materials to observe the effect of vernalization on flowering by different vernalization days.Meanwhile,materials at seedling stage,jointing stage and heading stage were taken under long daylight(LD)and short daylight(SD)conditions,respectively,and transcriptome sequencing was performed to analyze the differential changes of photoperiodic genes.The results showed that the heading and flowering time of D18 was later than that of K1 8 under different vernalization days.Under LD conditions,the expression of HvVRN1 in D18 was lower than that in K1 8 at jointing stage,so the heading and flowering time of D1 8 was later than that of K1 8.Under SD conditions,although the expression of HvVRN1 in D18 was lower than that in K18 at heading stage,the expression of HvPPD-H2 in D18 was higher than that in K18 at heading stage,resulting in the expression of HvVRN3 in D18 being higher than that in K1 8 at heading stage,so the heading and flowering time of D18 was earlier than that of K1 8 under SD conditions.