Breeding and Resistance Identification of Rice Line Qiangeng 187 Resistant to ALS Inhibitor Herbicides
At present,the most efficient chemical weed control techniques for paddy fields are ALS inhibitor herbicides. Two resistant rice varieties (lines),Jingemg 818 and Qiangeng 187,were used as materials,and Xudao No.3 was used as the sensitive control. After being treated with imidazole ethylnicotinic acid,the relative expression of target genes was analyzed. The results showed that the relative expression of target genes in the resistant materials gradually decreased to about 60% of the normal level. The results of the in vitro enzyme activity experiment showed that the resistance of Jingeng 818 and Qiangeng 187 with Ser-653-Asn amino acid substitution in the ALS to imidazole acenicotinic acid was more than 1000 times that of Xudao No.3.