A subsection control method of microfluidic automatic sampling in algal cell microscopic imaging
The rapid and automatic acquisition of microscopic images of algae cells has important application value.To address the issues of sample injection efficiency and cell imaging quality in microfluidic-microscopic imaging technology,a method for detecting the average flow rate of samples based on the duration of algal cells passing through the fluorescence detection window is studied,and a segmented control method of microfluidic auto-injection based on volume flow regulation is proposed.The results show that the average flow rate detection error of the sample is less than 5%when the sampling speed is within the range of 10-30 μL/min,and the segmented control realizes the automatic acquisition of high-quality microscopic images for algae cells.The quality of the microscopic images is basically consistent with that of microscopy,while the sample injection speed is increased by more than 68%.The research results provide an effective and practical method for efficient and automatic acquisition of microscopic images of algae cells.
万方数据
维普
