Effect of Microplastic Polypropylene and Cadmium on Testicles of Mice
Objective To investigate the effects of microplastic polypropylene and cadmium on reproductive damage in male mice,and to explore the mechanism of oxidative stress and inflammatory response in testicular tissue.Method Adopting 2x2 Factorial design,32 male healthy 6-week-old C57BU6 mice were randomly divided into control group(normal feed and drinking water),PP group(with 1％PP added to the feed),Cd group(with 1 mL/L Cd added to the drinking water),and PP+Cd group(with 1％PP added to the feed and 1 mL/L Cd added to the drinking water).After 5 weeks of feeding,the sperm quality of each group of mice was tested using the WLJY-9000 sperm quality detection system.The activity of SOD,GSH-Px,and MDA content in testicular tissue were measured using chemical colorimetry,IL-1β,IL-6 and TNF-α were detected using ELISA method.Results Compared with the control group,the PP group,Cd group,and PP+Cd group showed a decrease in sperm count and motility,an increase in total deformity rate,a de-crease in oxidative stress kinase SOD and GSH-PX activity in testicular tissue,an increase in stress product MDA content and inflam-matory factor IL-1β,IL-6 and TNF-α.All indicators among the groups showed statistical significance(P＜0.05)through analysis of variance;The levels of various indicators in the PP+Cd group were between the PP group and the Cd group,and after 2x2 analysis of the combined effects of factorial design,there was an interactive effect(P＜0.05)on various indicators of testicular toxicity in mice af-ter the combination of PP and Cd exposure,manifested as an antagonistic effect.Conclusion PP and Cd have a reproductive damage effect on male mice,and its mechanism is related to oxidative damage and inflammatory reactions in testicular tissue;When PP and Cd act together,PP can reduce the damage effect of Cd.
万方数据
维普
