GC-MS Component Analysis and Antibacterial Activity Comparison of Volatile Oil from Different Parts of Ocimum Basilicum from Southern Fujian
Objective To analyze the components and compare the antibacterial activity of volatile oil from different parts of Ocimum basi-licum in southern Fujian.Methods The volatile oils from different parts(seeds,leaves,roots and stems)of Ocimum basilicum in south-ern Fujian were extracted by steam distillation,and their chemical constituents were identified by gas chromatography-mass spectrometry(GC-MS).Staphylococcus aureus and Escherichia coli were used as identification bacteria,and the in vitro antibacterial activity of vola-tile oil from four parts of Ocimum basilicum was compared by the filter paper diffusion method.Result A total of 30,26,16 and 26 vola-tile compounds were identified from the seeds,leaves,roots and stems of Ocimum basilicum in southern Fujian,respectively.The antibac-terial activity of seeds and leaves was significant,and the antibacterial activity of roots and stems was weak.Conclusion The seeds and leaves of Ocimum basilicum are similar in chemical composition and main component content,and show comparable antibacterial activity against Staphylococcus aureus and Escherichia coli.The antibacterial activity of volatile oil from roots and stems is weak.
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