Effect of Tanshinone ⅡA on Viability and Apoptosis of Human Gastric Cancer BGC-823 Cells
Objective In this study,the effects of different concentrations of tanshinone ⅡA on the viability of gastric cancer BGC-823 cells were observed,and the mechanism of tanshinone ⅡA promoting the apoptosis of BGC-823 cells was investigated. Methods BGC-823 cells at logarithmic growth stage were cultured with 0.5,1.0,2.0 and 5.0 mg/L tanshinone ⅡA,respectively,for 12 hours.The mor-phological changes of BGC-823 cells were observed by optical microscope,and the inhibitory effect of tanshinone ⅡA on cell viability was detected by MTT assay. Flow cytometry was used to detect apoptosis rate. The expressions of apoptosis-related proteins Bcl-2,Bax and Cleaved-Caspase-3 were detected by Western blot. Results Light microscopy showed that different concentrations of tanshinone ⅡA could lead to changes in cell morphology. The MTT assay demonstrated a significant inhibition of BGC-823 cell viability following 12-hour treatment with tanshinone ⅡA in a dose-dependent manner(P<0.05 or P<0.01).Flow cytometry demonstrated that following a 12-hour treatment of tanshinone ⅡA on BGC-823 cells,the proportions of early apoptosis in each concentration group were(5.80±0.32)％(P<0.05),(7.90±0.43)％(P<0.05),(10.20±0.42)％(P<0.01),and(20.44±1.24)％(P<0.01),respectively. The results of the Western blot assay demonstrated that following the treatment of BGC-823 cells with various concentrations of tanshinone ⅡA for a duration of 12 hours,there was a tendency for the expression levels of Bax and Cleaved Caspase-3 protein in the experimental group to be up-regulated,in comparison to the control group. Similarly,the expression levels of Bcl-2 protein appeared to be down-regulated. Conclusions Tan-shinone ⅡA inhibits BGC-823 cell viability and promotes apoptosis in a dose-dependent manner.
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