Correlation of Streptozotocin and High-Fat Diet-Induced Polarization of Splenic Macrophages in Diabetic Mice
Objective To explore the effects of streptozotocin(STZ)and high-fat diet on inflammatory cytokines in serum and polariza-tion phenotype of splenic macrophages in diabetes model mice under different conditions. Methods C57BL/6J mice were selected and divided into the control group and the experimental group. The control group was fed with normal mouse feed,and the experimental group was divided into a pure high-fat feeding group and a high-fat feeding combined with STZ drug group. The combined group was given STZ concentrations of 1.25,2.5,and 5 g/L. The experimental period was 8 weeks,and the state,weight,and blood glucose values of the mice were observed and recorded;ELISA detection of serum interleukin-1(IL-1),IL-6,and tumor necrosis factor-α(TNF-α);Flow cytom-etry was used to detect the expression of CD40,CD86,and CD206 in splenic macrophages,as well as the level of reactive oxygen species (ROS). Results Compared with the control group,diet induction and diet combined with STZ significantly increased the levels of serum IL-1,IL-6,and TNF-αin mice;the expression of CD40 and CD86 increased,while the expression of CD206 decreased;The ROS level significantly increased. Conclusion The level of inflammatory cytokines in diabetes model mice is elevated;The level of reactive oxygen species in splenic macrophages increased and polarized towards M1.
万方数据
维普
