Study on Quality Control Method of Huangqi Guizhi Wuwu Granules
Objective To establish a quality control method of Huangqi Guizhi Wuwu Granules(HGWG),and provide a basis for the qual-ity control research of the preparation.Methods Astragali Radix and Paeoniae Radix Alba in HGWG were qualitatively identified by thin layer chromatography;The particle size,water content and solubility of HGWG granules were examined by the method of general prin-ciples of Chinese Pharmacopoeia(VolumeⅣ);The content of calycosin-7-O-β-D-glucoside in HGWG was determined by high perfor-mance liquid chromatography.Results In this study,the qualitative identification methods of Astragali Radix and Paeoniae Radix Alba in HGWG were established,the spots were clear,and the negative control had no interference;The results of particle size,water content and solubility of HGWG were in line with the requirements of Chinese Pharmacopoeia standards;The linear range of calycosin-7-O-β-D-glucoside was 5-250 mg/L(r=0.999 4),the average recovery was 98.9％and the content range of calycosin-7-O-β-D-glucoside in 6 batches of HGWG was 0.065 9-0.077 0 mg/g.Conclusion The thin layer chromatography method,preparation examination method and high performance liquid chromatography method used in this study have strong specificity and good repeatability,which can provide a ba-sis for the quality control of HGWG.
万方数据
维普
