Effects of Curcumin on Lipid Toxicity Injury and Surface Markers of Umbilical Cord Mesenchymal Stem Cells
Objective To investigate the effects of palmitic acid on cord mesenchymal stem cells(MSCs)injury,and to analyze the ef-fects of curcumin on lipid toxicity and surface markers of stem cells.Methods On the basis of cultured MSCs in vitro,a palmitic acid-induced cell lipid oxidation model was established to screen the appropriate concentration of curcumin to protect the cells from lipid oxi-dation.Cell activity was detected by Trypan blue rejection method,and reactive oxygen species and mitochondrial membrane potential were detected by fluorescent dye labelling and flow cytometry.The expression of MSCs surface markers was detected by flow cytometry af-ter fluorescent antibody labeling.Results The isolated MSCs did not express CD45,CD79a,CD34,CD14 and HLA Ⅰ,and the positive rates of CD 105,CD73 and CD90 were 97.8％,100％and 100％,respectively.After 24 h treatment with control group,75 µmol/L,150 μmol/L and 300 μmol/L palmitate,the percentage of viable cells was(95.4±4.7)％,(89.6±5.2)％,(85.2±6.6)％and(76.1±5.8)％,respectively.The percentage of living cells decreased gradually.The cell viability of control group,palmitic acid group(75 µmol/L),cur-cumin group(7.5 μmol/L)and combined treatment group was measured,and the percentage of viable cells was(95.3±6.6)％,(84.6±7.1)％,(95.3±5.8)％and(90.3±7.2)％,respectively.Low concentrations of curcumin itself had no effect on cell viability and mitochon-dria,but had inhibitory effects on ROS triggered by palmitic acid and prevented the effect of palmitic acid on mitochondrial membrane po-tential.The protective effect of ROS on cell viability may be related to its reduction of mitochondrial damage.The expression of CD 105 and CD73 decreased after palmitic acid treatment,but curcumin could restore the expression of CD 105 and CD73 and stabilize their phe-notypes.Conclusion Palmitic acid can damage MSCs by stimulating ROS,decrease cell viability,and affect the expression of CD 105 and CD73 markers on MSCs surface.Curcumin can reduce ROS induced by palmitic acid,protect cell viability,stabilize linear body membrane potential,and restore the expression of MSCs surface markers CD 105 and CD73.
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