摘要
目的 为研究昆明山海棠（THH）的毒理作用。方法 使用流式细胞仪检测发现THH均可诱导Jurkat、CHE和NIH3T3细胞株微核形成和细胞凋亡。结果 3个细胞株表现出完全相似的变化规律，但Jurkat淋巴瘤细胞更为敏感，仅25 μl/皿即在各时相点出现微核形成高峰，50 μl/皿即出现细胞凋亡高峰。结论 THH对Jurkat肿瘤细胞比对CHE和NIH3T3细胞具有更大的细胞毒性。
Abstract
Objective To study the toxicity of Tripterygium Hypolaucun (Level) Hutch (THH). Methods The flow cytometry technique was used to measure the micronucleus (MN) formation and cell apoptosis of Jurkat, CHE and NIH3T3 cell lines induced by THH. Results THH could induce micronucleus formation and cell apoptosis of Jurkat, CHE and NIH3T3 cell lines. Quite similar changes were observed in three cell lines, but the Jurkat cells showed more sensitive than others. It was only 25 μl/plate THH that induced the high peak of MN formation at various time points, and only 50 μl/plate that induced the high peak of cell apoptosis. Conclusion The result indicates that THH has stronger toxicity to Jurkat cells than to CHE and NIH3T3 cells.
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维普
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