首页|五种水螅基因组DNA的RAPD多态性研究

五种水螅基因组DNA的RAPD多态性研究

GENETIC DNA DIVERSITY OF FIVE SPECIES OF HYDRAS

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利用RAPD技术对水螅科2属5种水螅进行了基因组DNA多态性研究.80个随机引物筛选出18个引物,共扩增出303个清晰稳定的DNA片段,分子量在0.3～3.0kb之间.以UPGMA聚类法构建树状图,阐明水螅属种的分类关系:大庆水螅Hydra daqingensis、北京水螅H.beijingensis和强壮水螅H.robusta 3种分成两组并聚在一起;不同属的漠河水螅H.mohensis和绿水螅Chlorohydra sp.聚为一支;由此2属5种水螅分为两个支系.
RAPD (random amplified polymorphic DNA) technique was applied to assess the genetic diversity among five species belonging to two genera of Hydraridae. Eighteen primers were selected from 80 random primers and yielded a total of 303 clear bands ranging from 0.3-3.0 kb. The classification relationships of hydras were clarified using UPGMA. Individuals of the same species clustered respectively first. Hydra daqingensis and H. beijingensis showed close relationship with H. robusta , H. mo-hensis and Chlorohydra sp. belonging to different genera showed close relationship, so five species of hydras could be divided into two groups.

Hydra daqingenstsH. beijingensisH. robustaH. mohensisChlorohydra sp., RAPD

范学铭、肖玮、张彦帅

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哈尔滨师范大学生命与环境科学学院生物学系,哈尔滨,150080

大庆水螅 北京水螅 强壮水螅 漠河水螅 绿水螅 RAPD

黑龙江省自然科学基金黑龙江省教育厅自然科学基金

2005

动物分类学报
中国科学院动物研究所,中国动物学会,中国昆虫学会

动物分类学报

CSCD北大核心
影响因子：0.195
ISSN：1000-0739
年,卷(期)：2005.30(1)
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