首页|Outlining key inflammation-associated parameters during early phase of an experimental gram-negative sepsis model in rhesus macaques (Macaca mulatta)
Outlining key inflammation-associated parameters during early phase of an experimental gram-negative sepsis model in rhesus macaques (Macaca mulatta)
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The aim of this study was to identify inflammation-associated markers during the early phase of sepsis in rhesus macaque. Four rhesus macaques were given an in-travenous dose of 1010 CFU/kg of E. coli. Blood samples were collected before, or 30 minutes, 2, 4, 6 and 8 hours after E. coli infusion. Physiological parameters, bac-teremia, endotoxemia, C-reactive protein (CRP), procalcitonin (PCT), and plasma cy-tokines/chemokines were determined for each animal. Bacteremia was present in all animals from 30 minutes to 3 hours after E. coli infusion whereas endotoxin was de-tected during the full-time course. CRP and PCT levels remained at detectable levels during the whole experimental window suggesting an ongoing inflammatory process. Signature cytokines and chemokines such as TNF-α, MIP-1α, and MIP-1β peaked about 2 hours after E. coli infusion and decreased thereafter. Plasma IL-6, IL-12p40, IFN-γ, and IL-1Ra, as well as I-TAC, MIG, IP-10 and MCP-1, remained at detectable levels after 4 hours of E. coli infusion. This nonhuman primate model could be useful for the assessment of new therapeutics aiming to suppress key inflammatory markers throughout sepsis early phases.
chemokinescytokinesMacaca mulattasepsis
Jose J. Rosado-Franco、Marcos J. Ramos-Benitez、Laura M. Parodi、Derick Rosario、Nicole Compo、Luis D. Giavedoni、Ana M. Espino
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Department of Microbiology and Medical Zoology, Medical Sciences Campus, University of Puerto Rico, San Juan, Puerto Rico
Southwest National Primate Research Center, Texas Biomedical Research Institute,San Antonio, TX, USA
Chemistry Department, Rio Piedras Campus, University of Puerto Rico, San Juan, Puerto Rico
Medical Sciences Campus, Caribbean Primate Research Center University of Puerto Rico, Toa Baja, Puerto Rico
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Office of Research Infrastructure Program of NIH (ORIP-NIH) 2016-2021National Institute on Minority Health and Health DisparitiesNational Institute on Minority Health and Health DisparitiesNational Institute on Minority Health and Health DisparitiesSouthwest National Primate Research Centre
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