首页|Neural stem cell-derived exosomes regulate cell proliferation,migration,and cell death of brain microvascular endothelial cells via the miR-9/Hes1 axis under hypoxia

Neural stem cell-derived exosomes regulate cell proliferation,migration,and cell death of brain microvascular endothelial cells via the miR-9/Hes1 axis under hypoxia

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
Background:Our previous study found that mouse embryonic neural stem cell(NSC)-derived exosomes(EXOs)regulated NSC differentiation via the miR-9/Hes1 axis.However,the effects of EXOs on brain microvascular endothelial cell(BMEC)dysfunction via the miR-9/Hes1 axis remain unknown.Therefore,the current study aimed to determine the effects of EXOs on BMEC proliferation,migration,and death via the miR-9/Hes1 axis.Methods:Immunofluorescence,quantitative real-time polymerase chain reaction,cell counting kit-8 assay,wound healing assay,calcein-acetoxymethyl/propidium iodide staining,and hematoxylin and eosin staining were used to determine the role and mechanism of EXOs on BMECs.Results:EXOs promoted BMEC proliferation and migration and reduced cell death under hypoxic conditions.The overexpression of miR-9 promoted BMEC prolifera-tion and migration and reduced cell death under hypoxic conditions.Moreover,miR-9 downregulation inhibited BMEC proliferation and migration and also promoted cell death.Hes1 silencing ameliorated the effect of amtagomiR-9 on BMEC proliferation and migration and cell death.Hyperemic structures were observed in the regions of the hippocampus and cortex in hypoxia-induced mice.Meanwhile,EXO treatment improved cerebrovascular alterations.Conclusion:NSC-derived EXOs can promote BMEC proliferation and migra-tion and reduce cell death via the miR-9/Hes1 axis under hypoxic conditions.Therefore,EXO therapeutic strategies could be considered for hypoxia-induced vascular injury.

brain microvascular endothelial cellsexosomesHes1miR-9neural stem cells

Xiaojun Deng、Xiaoyi Hu、Shang Wang、Hui Zhao、Yaqin Wei、Jiaqi Fu、Wenhui Wu、Jinming Liu、Caicai Zhang、Lili Wang、Ping Yuan

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Department of Critical Care Medicine,Shanghai Sixth People's Hospital,Shanghai Jiao Tong University,Shanghai,China

Department of Cardio-Pulmonary Circulation,Shanghai Pulmonary Hospital,School of Medicine,Tongji University,Shanghai,China

Institute of Bismuth Science,University of Shanghai for Science and Technology,Shanghai,China

Department of Physiology,Hainan Medical University Haikou,Hainan,China

Department of Clinical Medical Laboratory Center,Hangzhou Red Cross Hospital,Zhejiang,China

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Program of Natural Science Foundation of ShanghaiProgram of Natural Science Foundation of ShanghaiProgram of National Natural Science Foundation of China中央高校基本科研业务费专项Program of Shanghai Municipal Health CommissionProgram of National Key Research and Development Project of ChinaPujiang Talent ProgramProgram of Shanghai Sixth People's Hospital Level Scientific Research FundProgram of Shanghai Sixth People's Hospital Level Scientific Research FundProgram of Shanghai Pulmonary HospitalProgram of Shanghai Pulmonary HospitalProgram of Shanghai Pulmonary Hospital

21ZR145380022ZR1452400823700572212022056220204Y03842023YFC250950022PJD064DY2019003ynhg202123FKLY20005fkzr2320fk18003

2024

10.1002/ame2.12394

动物模型与实验医学(英文)
中国实验动物学会,中国医学科学院医学实验动物研究所

动物模型与实验医学(英文)

ISSN：2096-5451
年,卷(期)：2024.7(1)
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