首页|Identifying genetic susceptibility to Aspergillus fumigatus infection using collaborative cross mice and RNA-Seq approach

Identifying genetic susceptibility to Aspergillus fumigatus infection using collaborative cross mice and RNA-Seq approach

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  • 国家科技期刊平台
  • NETL
  • NSTL
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Background:Aspergillus fumigatus(Af)is one of the most ubiquitous fungi and its infection potency is suggested to be strongly controlled by the host genetic back-ground.The aim of this study was to search for candidate genes associated with host susceptibility to Aspergillus fumigatus(Af)using an RNAseq approach in CC lines and hepatic gene expression.Methods:We studied 31 male mice from 25 CC lines at 8 weeks old;the mice were infected with Af.Liver tissues were extracted from these mice 5 days post-infection,and next-generation RNA-sequencing(RNAseq)was performed.The GENE-E analysis platform was used to generate a clustered heat map matrix.Results:Significant variation in body weight changes between CC lines was ob-served.Hepatic gene expression revealed 12 top prioritized candidate genes differ-entially expressed in resistant versus susceptible mice based on body weight changes.Interestingly,three candidate genes are located within genomic intervals of the previ-ously mapped quantitative trait loci(QTL),including Gm16270 and Stox1 on chromo-some 10 and Gm11033 on chromosome 8.Conclusions:Our findings emphasize the CC mouse model's power in fine mapping the genetic components underlying susceptibility towards Af.As a next step,eQTL analysis will be performed for our RNA-Seq data.Suggested candidate genes from our study will be further assessed with a human cohort with aspergillosis.

aspergillus fumigatus infectioncollaborative cross(CC)micegene expression profilegene-networkhost susceptibilityquantitative trait loci(QTL)mappingRNA-Seq

Roa'a H.S.Yosief、Iqbal M.Lone、Aharon Nachshon、Heinz Himmelbauer、Irit Gat-Viks、Fuad A.Iraqi

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Department of Clinical Microbiology and Immunology,Sackler Faculty of Medicine,Tel-Aviv University,Tel-Aviv,Israel

School of Molecular Cell Biology and Biotechnology,George S.Wise Faculty of Life Sciences,Tel Aviv University,Tel Aviv,Israel

Institute of Computational Biology,Department of Biotechnology,University of Natural Resources and Life Sciences,Muthgasse 18,1190 Vienna,Austria

European Sequencing and Genotyping Institutes(ESGI)European Sequencing and Genotyping Institutes(ESGI)European Sequencing and Genotyping Institutes(ESGI)Tel-Aviv University(TAU)

075491/Z/04085906/Z/08/Z090532/Z/09/Z

2024

10.1002/ame2.12386

动物模型与实验医学(英文)
中国实验动物学会,中国医学科学院医学实验动物研究所

动物模型与实验医学(英文)

ISSN：2096-5451
年,卷(期)：2024.7(1)
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