首页|Simple procedure for assessing diffuse subarachnoid hemorrhage successfully created using filament perforation method in mice

Simple procedure for assessing diffuse subarachnoid hemorrhage successfully created using filament perforation method in mice

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  • 国家科技期刊平台
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The murine model of subarachnoid hemorrhage(SAH)is a valuable experimental tool for investigating molecular and cellular mechanisms,and the endovascular filament perforation technique can be used to simulate prominent pathophysiological features observed after human SAH;however,current validation methods for assessing an appropriate SAH model are limited.Here,we introduce a simple procedure for se-lecting a mouse model of diffuse SAH.SAH was induced in 24 mice using a standard filament perforation method.After confirming survival at 24 h,SAH was scored 0-1 based on T2*-weighted images on whole-brain magnetic resonance imaging(MRI)and visual surveillance of the cisterna magna(CM)through the dura mater.The CM-based SAH grading correlated well with a reference parameter defined by extracted brain(r 2 = 0.53,p<0.0001).The receiver operating characteristic curve revealed a sensi-tivity of 85%and a specificity of 91%for detecting diffuse SAH,with a similar area under the curve(0.89±0.06[standard error of the mean])as the MRI-based grading(0.72±0.10,p = 0.12).Our data suggest that confirming an SAH clot in the CM is a valuable way to select a clinically relevant diffuse SAH model that can be used in future experimental studies.

cisterna magnaclot distributionfilament perforationmouse modelsubarachnoid hemorrhage

Tatsushi Mutoh、Ryota Tochinai、Hiroaki Aono、Masayoshi Kuwahara、Yasuyuki Taki、Tatsuya Ishikawa

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Department of Surgical Neurology,Research Institute for Brain and Blood Vessels,Akita Cerebrospinal and Cardiovascular Center,Akita,Japan

Department of Aging Research and Geriatric Medicine,Institute of Development,Aging and Cancer,Tohoku University,Sendai,Japan

Department of Veterinary Pathophysiology and Animal Health,Graduate School of Agriculture and Life Sciences,The University of Tokyo,Tokyo,Japan

日本学术振兴会项目

22K09110

2024

10.1002/ame2.12372

动物模型与实验医学(英文)
中国实验动物学会,中国医学科学院医学实验动物研究所

动物模型与实验医学(英文)

ISSN：2096-5451
年,卷(期)：2024.7(1)
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