首页|Jiaohong pills attenuate neuroinflammation and amyloid-β protein-induced cognitive deficits by modulating the mitogen-activated protein kinase/nuclear factor kappa-B pathway

Jiaohong pills attenuate neuroinflammation and amyloid-β protein-induced cognitive deficits by modulating the mitogen-activated protein kinase/nuclear factor kappa-B pathway

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Background:Jiaohong pills (JHP) consist of Pericarpium Zanthoxyli (PZ) and Radix Rehmanniae ,two herbs that have been extensively investigated over many years due to their potential protective effects against cognitive decline and memory im-pairment.However,the precise mechanisms underlying the beneficial effects remain elusive.Here,research studies were conducted to investigate and validate the thera-peutic effects of JHP on Alzheimer's disease.Methods:BV-2 cell inflammation was induced by lipopolysaccharide.AD mice were administered amyloid-β (Aβ).Behavioral experiments were used to evaluate learn-ing and memory ability.The levels of nitric oxide (NO),tumor necrosis factor-alpha (TNF-α),interleukin-1β (IL-1β),and interleukin-10 (IL-10) were detected using enzyme-linked immunosorbent assay (ELISA).The protein expressions of inducible nitric oxide synthase (iNOS) and the phosphorylation level of mitogen-activated protein kinase (MAPK) and nuclear factor kappa-B (NF-κB) were detected using Western blot.Nissl staining was used to detect neuronal degeneration.Results:The results demonstrated that an alcoholic extract of PZ significantly de-creased the levels of NO,IL-1β,TNF-α,and iNOS; increased the expression level of IL-10; and significantly decreased the phosphorylation levels of MAPK and NF-κB.These inhibitory effects were further confirmed in the AD mouse model.Meanwhile,JHP improved learning and memory function in AD mice,reduced neuronal damage,and enriched the Nissl bodies in the hippocampus.Moreover,IL-1β and TNF-α in the cortex were significantly downregulated after JHP administration,whereas IL-10 showed increased expression.Conclusions:It was found that JHP reduced neuroinflammatory response in AD mice by targeting the MAPK/NF-κB signaling pathway.

amyloid-β (Aβ) proteinBV2neuroinflammationPericarpium ZanthoxyliRadix Rehmanniae

Hong Zhang、Weiyan Cai、Lijinchuan Dong、Qing Yang、Qi Li、Qingsen Ran、Li Liu、Yajie Wang、Yujie Li、Xiaogang Weng、Xiaoxin Zhu、Ying Chen

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Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing,China

Scientific and Technological Innovation Project of the Chinese Academy of Chinese Medical SciencesScientific and Technological Innovation Project of the Chinese Academy of Chinese Medical SciencesScientific and Technological Innovation Project of the Chinese Academy of Chinese Medical SciencesKey Technology Research Foundation of the National Institutes for Food and Drug ControlNational Natural Science Foundation of China

CI2023E001TS02CI2021A04905CI2021B015GJJS-2022-7-182074103

2024

10.1002/ame2.12369

动物模型与实验医学(英文)
中国实验动物学会,中国医学科学院医学实验动物研究所

动物模型与实验医学(英文)

ISSN：2096-5451
年,卷(期)：2024.7(3)
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