CircPMS1 promotes proliferation of pulmonary artery smooth muscle cells,pulmonary microvascular endothelial cells,and pericytes under hypoxia

Xiaoyi Hu ¹Shang Wang ¹Hui Zhao ²Yaqin Wei ³Ruowang Duan ⁴Rong Jiang ¹Wenhui Wu ¹Qinhua Zhao ¹Sugang Gong ¹Lan Wang ¹Jinming Liu ¹Ping Yuan¹

扫码查看

作者信息

1. Department of Cardio-Pulmonary Circulation,Shanghai Pulmonary Hospital,School of Medicine,Tongji University,Shanghai,China
2. Department of Cardio-Pulmonary Circulation,Shanghai Pulmonary Hospital,School of Medicine,Tongji University,Shanghai,China;Institute of Bismuth Science,University of Shanghai for Science and Technology,Shanghai,China
3. Department of Geriatrics,Shanghai Institute of Geriatrics,Huadong Hospital,Fudan University,Shanghai,China
4. Department of Anesthesiology,Shanghai Pulmonary Hospital,School of Medicine,Tongji University,Shanghai,China
折叠

Abstract

Background:Circular RNAs (circRNAs) have been recognized as significant regulators of pulmonary hypertension (PH); however,the differential expression and function of circRNAs in different vascular cells under hypoxia remain unknown.Here,we identi-fied co-differentially expressed circRNAs and determined their putative roles in the proliferation of pulmonary artery smooth muscle cells (PASMCs),pulmonary micro-vascular endothelial cells (PMECs),and pericytes (PCs) under hypoxia.Methods:Whole transcriptome sequencing was performed to analyze the differential expression of circRNAs in three different vascular cell types.Bioinformatic analysis was used to predict their putative biological function.Quantitative real-time polymer-ase chain reaction,Cell Counting Kit-8,and EdU Cell Proliferation assays were carried out to determine the role of circular postmeiotic segregation 1 (circPMS1) as well as its potential sponge mechanism in PASMCs,PMECs,and PCs.Results:PASMCs,PMECs,and PCs exhibited 16,99,and 31 differentially expressed circRNAs under hypoxia,respectively.CircPMS1 was upregulated in PASMCs,PMECs,and PCs under hypoxia and enhanced the proliferation of vascular cells.CircPMS1 may upregulate DEP domain containing 1 (DEPDC1) and RNA polymerase Ⅱ subunit D expression by targeting microRNA-432-5p (miR-432-5p) in PASMCs,upregulate MAX interactor 1 (MXI1) expression by targeting miR-433-3p in PMECs,and upregulate zinc finger AN1-type containing 5 (ZFAND5) expression by targeting miR-3613-5p in PCs.Conclusions:Our results suggest that circPMS1 promotes cell proliferation through the miR-432-5p/DEPDC1 or miR-432-5p/POL2D axis in PASMCs,through the miR-433-3p/MXI1 axis in PMECs,and through the miR-3613-5p/ZFAND5 axis in PCs,which provides putative targets for the early diagnosis and treatment of PH.

Key words

circular postmeiotic segregation 1/circular RNAs/hypoxia/pulmonary hypertension/vascular cells

引用本文复制引用

基金项目

Program of Natural Science Foundation of Shanghai(21ZR1453800)

Program of Natural Science Foundation of Shanghai(201409004100)

Fundamental Research Funds for the Central Universities(22120220562)

Program of National Natural Science Foundation of China(81870044)

Program of Shanghai Pulmonary Hospital(FKLY20005)

Program of Shanghai Pulmonary Hospital(fkzr2320)

出版年

2024

动物模型与实验医学(英文)

中国实验动物学会,中国医学科学院医学实验动物研究所

动物模型与实验医学(英文)

ISSN：2096-5451

参考文献量2

段落导航