首页|CircPMS1 promotes proliferation of pulmonary artery smooth muscle cells,pulmonary microvascular endothelial cells,and pericytes under hypoxia

CircPMS1 promotes proliferation of pulmonary artery smooth muscle cells,pulmonary microvascular endothelial cells,and pericytes under hypoxia

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Background:Circular RNAs (circRNAs) have been recognized as significant regulators of pulmonary hypertension (PH); however,the differential expression and function of circRNAs in different vascular cells under hypoxia remain unknown.Here,we identi-fied co-differentially expressed circRNAs and determined their putative roles in the proliferation of pulmonary artery smooth muscle cells (PASMCs),pulmonary micro-vascular endothelial cells (PMECs),and pericytes (PCs) under hypoxia.Methods:Whole transcriptome sequencing was performed to analyze the differential expression of circRNAs in three different vascular cell types.Bioinformatic analysis was used to predict their putative biological function.Quantitative real-time polymer-ase chain reaction,Cell Counting Kit-8,and EdU Cell Proliferation assays were carried out to determine the role of circular postmeiotic segregation 1 (circPMS1) as well as its potential sponge mechanism in PASMCs,PMECs,and PCs.Results:PASMCs,PMECs,and PCs exhibited 16,99,and 31 differentially expressed circRNAs under hypoxia,respectively.CircPMS1 was upregulated in PASMCs,PMECs,and PCs under hypoxia and enhanced the proliferation of vascular cells.CircPMS1 may upregulate DEP domain containing 1 (DEPDC1) and RNA polymerase Ⅱ subunit D expression by targeting microRNA-432-5p (miR-432-5p) in PASMCs,upregulate MAX interactor 1 (MXI1) expression by targeting miR-433-3p in PMECs,and upregulate zinc finger AN1-type containing 5 (ZFAND5) expression by targeting miR-3613-5p in PCs.Conclusions:Our results suggest that circPMS1 promotes cell proliferation through the miR-432-5p/DEPDC1 or miR-432-5p/POL2D axis in PASMCs,through the miR-433-3p/MXI1 axis in PMECs,and through the miR-3613-5p/ZFAND5 axis in PCs,which provides putative targets for the early diagnosis and treatment of PH.

circular postmeiotic segregation 1circular RNAshypoxiapulmonary hypertensionvascular cells

Xiaoyi Hu、Shang Wang、Hui Zhao、Yaqin Wei、Ruowang Duan、Rong Jiang、Wenhui Wu、Qinhua Zhao、Sugang Gong、Lan Wang、Jinming Liu、Ping Yuan

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Department of Cardio-Pulmonary Circulation,Shanghai Pulmonary Hospital,School of Medicine,Tongji University,Shanghai,China

Institute of Bismuth Science,University of Shanghai for Science and Technology,Shanghai,China

Department of Geriatrics,Shanghai Institute of Geriatrics,Huadong Hospital,Fudan University,Shanghai,China

Department of Anesthesiology,Shanghai Pulmonary Hospital,School of Medicine,Tongji University,Shanghai,China

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Program of Natural Science Foundation of ShanghaiProgram of Natural Science Foundation of ShanghaiFundamental Research Funds for the Central UniversitiesProgram of National Natural Science Foundation of ChinaProgram of Shanghai Pulmonary HospitalProgram of Shanghai Pulmonary Hospital

21ZR14538002014090041002212022056281870044FKLY20005fkzr2320

2024

10.1002/ame2.12332

动物模型与实验医学(英文)
中国实验动物学会,中国医学科学院医学实验动物研究所

动物模型与实验医学(英文)

ISSN：2096-5451
年,卷(期)：2024.7(3)
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