Abstract
Background:Severe trauma is associated with systemic inflammation and organ dys-function.Preclinical rodent trauma models are the mainstay of postinjury research but have been criticized for not fully replicating severe human trauma.The aim of this study was to create a rat model of multicompartmental injury which recreates profound traumatic injury.Methods:Male Sprague-Dawley rats were subjected to unilateral lung contusion and hemorrhagic shock (LCHS),multicompartmental polytrauma (PT) (unilateral lung con-tusion,hemorrhagic shock,cecectomy,bifemoral pseudofracture),or naïve controls.Weight,plasma toll-like receptor 4 (TLR4),hemoglobin,spleen to body weight ratio,bone marrow (BM) erythroid progenitor (CFU-GEMM,BFU-E,and CFU-E) growth,plasma granulocyte colony-stimulating factor (G-CSF) and right lung histologic injury were assessed on day 7,with significance defined as p values <0.05 (*).Results:Polytrauma resulted in markedly more profound inhibition of weight gain compared to LCHS (p=0.0002) along with elevated plasma TLR4 (p<0.0001),lower hemoglobin (p<0.0001),and enlarged spleen to body weight ratios (p=0.004).Both LCHS and PT demonstrated suppression of CFU-E and BFU-E growth compared to naïve (p<0.03,p<0.01).Plasma G-CSF was elevated in PT compared to both naïve and LCHS (p<0.0001,p=0.02).LCHS and PT demonstrated significant histologic right lung injury with poor alveolar wall integrity and interstitial edema.Conclusions:Multicompartmental injury as described here establishes a reproduc-ible model of multicompartmental injury with worsened anemia,splenic tissue en-largement,weight loss,and increased inflammatory activity compared to a less severe model.This may serve as a more effective model to recreate profound traumatic in-jury to replicate the human inflammatory response postinjury.
基金项目
National Institutes of Health()
维普
万方数据