首页|Identification of novel genes associated with atherosclerosis in Bama miniature pig

Identification of novel genes associated with atherosclerosis in Bama miniature pig

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Background:Atherosclerosis is a chronic cardiovascular disease of great concern.However,it is difficult to establish a direct connection between conventional small animal models and clinical practice.The pig's genome,physiology,and anatomy reflect human biology better than other laboratory animals,which is crucial for studying the pathogenesis of atherosclerosis.Methods:We used whole-genome sequencing data from nine Bama minipigs to per-form a genome-wide linkage analysis,and further used bioinformatic tools to filter and identify underlying candidate genes.Candidate gene function prediction was performed using the online prediction tool STRING 12.0.Immunohistochemistry and immunofluorescence were used to detect the expression of proteins encoded by can-didate genes.Results:We mapped differential single nucleotide polymorphisms (SNPs) to genes and obtained a total of 102 differential genes,then we used GO and KEGG pathway enrichment analysis to identify four candidate genes,including SLA-1 ,SLA-2 ,SLA-3 ,and TAP2 .nsSNPs cause changes in the primary and tertiary structures of SLA-I and TAP2 proteins,the primary structures of these two proteins have undergone amino acid changes,and the tertiary structures also show slight changes.In addition,im-munohistochemistry and immunofluorescence results showed that the expression changes of TAP2 protein in coronary arteries showed a trend of increasing from the middle layer to the inner layer.Conclusions:We have identified SLA-I and TAP2 as potential susceptibility genes of atherosclerosis,highlighting the importance of antigen processing and immune re-sponse in atherogenesis.

atherosclerosiscandidate genesgenome-wide linkage analysismajor histocompatibility complexwhole genome sequencing

Dengfeng Ding、Yuqiong Zhao、Yunxiao Jia、Miaomiao Niu、Xuezhuang Li、Xinou Zheng、Hua Chen

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Laboratory Animal Center,Chinese PLA General Hospital,Beijing,China

Special Scientific Research Project of Army Laboratory AnimalsNational Natural Science Foundation of China

SYDW[2020]0132370568

2024

10.1002/ame2.12412

动物模型与实验医学(英文)
中国实验动物学会,中国医学科学院医学实验动物研究所

动物模型与实验医学(英文)

ISSN：2096-5451
年,卷(期)：2024.7(3)
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