Preliminary study of metabonomic changes during the progression of atherosclerosis in miniature pigs

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外文摘要：Background:To explore potential biomarkers for early diagnosis of atherosclerosis (AS) and provide basic data for further research on AS,the characteristics of serum metabolomics during the progression of AS in mini-pigs were observed dynamically.Methods:An AS model in Bama miniature pigs was established by a high-cholesterol and high-fat diet.Fasting serum samples were collected monthly for metabolomics and serum lipid detection.At the end of the treatment period,pathological analysis of the abdominal aorta and coronary artery was performed to evaluate the lesions of AS,thereby distinguishing the susceptibility of mini-pigs to AS.The metabolomics was de-tected using a high-resolution untargeted metabolomic approach.Statistical analysis was used to identify metabolites associated with AS susceptibility.Results:Based on pathological analysis,mini-pigs were divided into two groups:a susceptible group (n=3) and a non-susceptible group ( n=6).A total of 1318 metabo-lites were identified,with significant shifting of metabolic profiles over time in both groups.Dynamic monitoring analysis highlighted 57 metabolites that exhibited an ob-vious trend of differential changes between two groups with the advance of time.The KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analysis in-dicated significant disorders in cholesterol metabolism,primary bile acid metabolism,histidine metabolism,as well as taurine and hypotaurine metabolism.Conclusions:During the progression of AS in mini-pigs induced by high-cholesterol/high-fat diet,the alterations in serum metabolic profile exhibited a time-dependent pattern,accompanied by notable disturbances in lipid metabolism,cholesterol me-tabolism,and amino acid metabolism.These metabolites may become potential bio-markers for early diagnosis of AS.

外文关键词：

atherosclerosismetabolomicsminiature pigsusceptibility

作者：

Yunxiao Jia、Yuqiong Zhao、Miaomiao Niu、Changqi Zhao、Xuezhuang Li、Hua Chen

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作者单位：

Laboratory Animal Center,Chinese PLA General Hospital,Beijing,People's Republic of China

基金：

Animal Center of the Military Medical Research Institute of the Military ScienceSpecial Scientific Research Project of Laboratory AnimalsSpecial Scientific Research Project of Laboratory AnimalsSpecial Scientific Research Project of Laboratory Animals

项目编号：

license number:SYXK[Jun]2017-0022SYDW[2018]14SYDW[2020]01SYDW-KY[2021]03

出版年：

2024

DOI：

10.1002/ame2.12462

动物模型与实验医学(英文)

中国实验动物学会,中国医学科学院医学实验动物研究所

动物模型与实验医学(英文)

ISSN：2096-5451

年,卷(期)：2024.7(4)