Influence of ear tags on the results of body composition analysis in mice

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外文摘要：Background:The aim of this study was to investigate the influence of marking meth-ods on the outcomes of body composition analysis and provide guidance for the se-lection of marking methods in mouse body composition analysis.Methods:Male C57BL/6J mice aged 6 weeks were randomly assigned for pre-and post-ear tagging measurements.The body composition of the mice was measured using a small animal body composition analyzer,which provided measurements of the mass of fat,lean,and free fluid.Then,the mass of fat,lean and free fluid to body weight ratio was gained.Further data analysis was conducted to obtain the range and coeffi-cient of variation in body composition measurements for each mouse.The distribution of fat and lean tissue in the mice was also analyzed by comparing the fat-to-lean ratio.Results:(1) The mass of all body composition components in the ear tagging group was significantly lower than that in the control group.(2) There was a significant in-crease in the range and coefficient of variation of body composition measurements between the ear tagging group and the control group.(3) The fat-to-lean ratio in the ear tagging group was significantly lower than that in the control group.Conclusions:Ear tagging significantly lowered the results of body composition analy-sis in mice and higher the results of measurement error.Therefore,ear tagging should be avoided as much as possible when conducting body composition analysis experi-ments in mice.

外文关键词：

body composition analysisear tagginglabeling methodstainless steeltoe clipping

作者：

He Liu、Yinghua Zhang、Peng Zhang、Weiping Teng、Zhongyan Shan、Yushu Li、Dan Wang

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作者单位：

Department of Endocrinology and Metabolism and the Institute of Endocrinology,NHC Key Laboratory of Diagnosis and Treatment of Thyroid Diseases,The First Hospital of China Medical University,Shenyang,China

School of Public Health China Medical University,China Medical University,Shenyang,China

Shenyang Food and Drug Inspection Institute,Shenyang,China

出版年：

2024

DOI：

10.1002/ame2.12450

动物模型与实验医学(英文)

中国实验动物学会,中国医学科学院医学实验动物研究所

动物模型与实验医学(英文)

ISSN：2096-5451

年,卷(期)：2024.7(4)