首页|The activation of adenosine monophosphate-activated protein kinase inhibits the migration of tongue squamous cell carcinoma cells by targeting Claudin-1 via epithelial-mesenchymal transition

The activation of adenosine monophosphate-activated protein kinase inhibits the migration of tongue squamous cell carcinoma cells by targeting Claudin-1 via epithelial-mesenchymal transition

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The activation of adenosine monophosphate-activated protein kinase inhibits the migration of tongue squamous cell carcinoma cells by targeting Claudin-1 via epithelial-mesenchymal transition
Background:The role of Claudin-1 in tongue squamous cell carcinoma(TSCC)metas-tasis needs further clarification,particularly its impact on cell migration.Herein,our study aims to investigate the role of Claudin-1 in TSCC cell migration and its underly-ing mechanisms.Methods:36 TSCC tissue samples underwent immunohistochemical staining for Claudin-1.Western blotting and immunofluorescence analyses were conducted to evaluate Claudin-1 expression and distribution in TSCC cells.Claudin-1 knockdown cell lines were established using short hairpin RNA transfection.Migration effects were assessed through wound healing assays.Furthermore,the expression of EMT-associated molecules was measured via western blotting.Results:Claudin-1 expression decreased as TSCC malignancy increased.Adenosine monophosphate-activated protein kinase(AMPK)activation led to increased Claudin-1 expression and membrane translocation,inhibiting TSCC cell migration and epithelial-mesenchymal transition(EMT).Conversely,Claudin-1 knockdown reversed these inhibitory effects on migration and EMT caused by AMPK activation.Conclusions:Our results indicated that AMPK activation suppresses TSCC cell migra-tion by targeting Claudin-1 and EMT pathways.

AMPKClaudin-1EMTmigrationtongue squamous cell carcinoma

Xin-Yue Zhou、Qiu-Ming Liu、Zhuang Li、Xia-Yang Liu、Qi-Wei Zhao、Yu Wang、Feng-Hua Wu、Gang Zhao、Rui Sun、Xiao-Hong Guo

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Department of Basic Medicine,Hubei University of Chinese Medicine,Wuhan,China

Hubei Shizhen Laboratory,Wuhan,Hubei,China

Sino-German Biomedical Center,Hubei University of Technology,Wuhan,China

Center of Applied Biotechnology,Wuhan Institute of Bioengineering,Wuhan,China

Department of Stomatology,Shanxi Bethune Hospital,Shanxi Academy of Medical Sciences,Tongji Shanxi Hospital,Third Hospital of Shanxi Medical University,Taiyuan,China

Department of Oral and Maxillofacial Surgery,Shanxi Provincial People's Hospital,Taiyuan,China

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AMPK Claudin-1 EMT migration tongue squamous cell carcinoma

2024

10.1002/ame2.12444

动物模型与实验医学(英文)
中国实验动物学会,中国医学科学院医学实验动物研究所

动物模型与实验医学(英文)

ISSN：2096-5451
年,卷(期)：2024.7(5)