Abstract
Background:Triple-negative breast cancer(TNBC),which is so called because of the lack of estrogen receptors(ER),progesterone receptors(PR),and human epidermal growth factor receptor 2(HER2)receptors on the cancer cells,accounts for 10％-15％of all breast cancers.The heterogeneity of the tumor microenvironment is high.However,the role of plasma cells controlling the tumor migration progression in TNBC is still not fully understood.Methods:We analyzed single-cell RNA sequencing data from five HER2 positive,12 ER positive/PR positive,and nine TNBC samples.The potential targets were validated by immunohistochemistry.Results:Plasma cells were enriched in TNBC samples,which was consistent with validation using data from The Cancer Genome Atlas.Cell communication analysis revealed that plasma cells interact with T cells through the intercellular adhesion mol-ecule 2-integrin-aLb2 complex,and then release interleukin 1 beta(IL1B),as verified by immunohistochemistry,ultimately promoting tumor growth.Conclusion:Our results revealed the role of plasma cells in TNBC and identified IL1B as a new prognostic marker for TNBC.
维普
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