Genetic polymorphism in a cultured population of the crested ibis Nipponia nippon
The genetic polymorphism of captive crested ibis Nipponia nippon was carried out by protein electrophoresis, RAPD and microsatellite DNA technology. The results showed no polymorphism in 4 blood protein loci including Pre, Alb, Pa and TI loci in 40 birds. Among 40 arbitrary primers, 35 primers yielded the same bands whereas 5 primers yielded polymorphic bands. The average band-sharing rate of RAPD bands in the 40 birds was 0.86. 22 pairs of microsatellite primers from fish, cow and chicken were used to analyze the genetic polymorphism of 30 crested ibises. Four pairs of microsatellite primers were effective for crested ibis, and 13 alleies were obtained. The mean heterozygosity, average PIC, average allelic number of 4 pairs of primers in birds was 0.3760, 0.3382 and 2.075, respectively. The data indicated that the genetic diversity of the cultured crested ibis population is still poor but has significantly increased since 1981.
Crested ibisNipponia nipponBlood protein lociRAPDMicrosatellite DNAPolymorphism
NETL
NSTL
万方数据
