Interactions among deep-sea mussels and their epibiotic and endosymbiotic chemoautotrophic bacteria:Insights from multi-omics analysis

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外文摘要：Endosymbiosis with Gammaproteobacteria is fundamental for the success of bathymodioline mussels in deep-sea chemosynthesis-based ecosystems.However,the recent discovery of Campylobacteria on the gill surfaces of these mussels suggests that these host-bacterial relationships may be more complex than previously thought.Using the cold-seep mussel(Gigantidas haimaensis)as a model,we explored this host-bacterial system by assembling the host transcriptome and genomes of its epibiotic Campylobacteria and endosymbiotic Gammaproteobacteria and quantifying their gene and protein expression levels.We found that the epibiont applies a sulfur oxidizing(SOX)multienzyme complex with the acquisition of soxB from Gammaproteobacteria for energy production and switched from a reductive tricarboxylic acid(rTCA)cycle to a Calvin-Benson-Bassham(CBB)cycle for carbon assimilation.The host provides metabolic intermediates,inorganic carbon,and thiosulfate to satisfy the materials and energy requirements of the epibiont,but whether the epibiont benefits the host is unclear.The endosymbiont adopts methane oxidation and the ribulose monophosphate pathway(RuMP)for energy production,providing the major source of energy for itself and the host.The host obtains most of its nutrients,such as lysine,glutamine,valine,isoleucine,leucine,histidine,and folate,from the endosymbiont.In addition,host pattern recognition receptors,including toll-like receptors,peptidoglycan recognition proteins,and C-type lectins,may participate in bacterial infection,maintenance,and population regulation.Overall,this study provides insights into the complex host-bacterial relationships that have enabled mussels and bacteria to thrive in deep-sea chemosynthetic ecosystems.

外文关键词：

BathymodiolineChemosynthesisCold seepDeep-sea adaptationSymbiosis

作者：

Yi-Tao Lin、Ting Xu、Jack Chi-Ho Ip、Yanan Sun、Ling Fang、Tiangang Luan、Yu Zhang、Pei-Yuan Qian、Jian-Wen Qiu

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作者单位：

Department of Biology,Hong Kong Baptist University,Hong Kong SAR,China

Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou,Guangdong 511458,China

Department of Ocean Science,Hong Kong University of Science and Technology,Hong Kong SAR,China

Instrumental Analysis&Research Center,Sun Yat-Sen University,Guangzhou,Guangdong 510875,China

State Key Laboratory of Biocontrol,School of Life Sciences,Sun Yat-Sen University,Guangzhou,Guangdong 510875,China

Institute of Environmental and Ecological Engineering,Guangdong University of Technology,Guangzhou,Guangdong 510006,China

College of Life Sciences and Oceanography,Shenzhen University,Shenzhen,Guangdong 518060,China

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基金：

Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)香港研究资助局项目广东省自然科学基金

项目编号：

GML2019ZD0409SMSEGL20SC02121010212020A1515011117

出版年：

2023

DOI：

10.24272/j.issn.2095-8137.2022.279

动物学研究

中国科学院昆明动物研究所中国动物学会

动物学研究

CSTPCDCSCD

影响因子：0.582

ISSN：0254-5853

年,卷(期)：2023.44(1)

参考文献量102